Evidence map›Paper›PMID 36219726›Full record

ArticleACS synthetic biology2022

Flux-Balance Analysis and Mobile CRISPRi-Guided Deletion of a Conditionally Essential Gene in

Kathryne C Ford, Joshua A M Kaste, Yair Shachar-Hill, Michaela A TerAvest

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Shewanella oneidensis: Biotechnological Application of Metal-Reducing Bacteria.Advances in biochemical engineering/biotechnology · 2025
    Review
  2. Mobile-CRISPRi as a powerful tool for modulatingApplied and environmental microbiology · 2024
    Article
  3. Article
  4. A common inducer molecule enhances sugar utilization by Shewanella oneidensis MR-1.Journal of industrial microbiology & biotechnology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kathryne C FordDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, United States.
Joshua A M KasteDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, United States.ORCID 0000-0003-1942-0315
Yair Shachar-HillDepartment of Plant Biology, Michigan State University, East Lansing, Michigan 48824, United States.
Michaela A TerAvestDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, United States.ORCID 0000-0002-5435-3587
Michigan State University · US

Funding

Plant Biotechnology for Health and SustainabilityT32GM110523 · NIGMS · MICHIGAN STATE UNIVERSITY · PI LAST, ROBERT LOUIS · 2014 to 2023
$2.2M
NIGMS NIH HHS T32 GM110523
6 · The paper itself

Abstract

Carbon-neutral production of valuable bioproducts is critical to sustainable development but remains limited by the slow engineering of photosynthetic organisms. Improving existing synthetic biology tools to engineer model organisms to fix carbon dioxide is one route to overcoming the limitations of photosynthetic organisms. In this work, we describe a pipeline that enabled the deletion of a conditionally essential gene from the

Indexed as

Genes, EssentialShewanellaCarbon DioxideGene DeletionRibulose-Bisphosphate CarboxylaseCarbon DioxideRibulose-Bisphosphate Carboxylasecarbon metabolismCRISPRiflux-balance analysisgenetic engineeringShewanella oneidensissynthetic biology

Identifiers

PMID36219726
PMCPMC9595118
OpenAlexW4304207149

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.