Evidence map›Paper›PMID 36219276›Full record

ArticleCellular and molecular life sciences : CMLS2022

Human embryonic stem cell-specific role of YAP in maintenance of self-renewal and survival.

Mu Seog Choe, Chang Min Bae, So Jin Kim, Seung Tack Oh, Yu Jin Kown, Won-Young Choi, Ho Jae Han, Kyung Min Baek, Woochul Chang, Joong Sun Kim and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 1 country.

Mu Seog Choe *College of Pharmacy, Research Institute of Pharmaceutical Sciences, Vessel-Organ Interaction Research Center (VOICE, MRC), Kyungpook National University, Daegu, South Korea.
Chang Min Bae *College of Pharmacy, Research Institute of Pharmaceutical Sciences, Vessel-Organ Interaction Research Center (VOICE, MRC), Kyungpook National University, Daegu, South Korea.
So Jin Kim *College of Pharmacy, Research Institute of Pharmaceutical Sciences, Vessel-Organ Interaction Research Center (VOICE, MRC), Kyungpook National University, Daegu, South Korea.
Seung Tack OhCollege of Pharmacy, Research Institute of Pharmaceutical Sciences, Vessel-Organ Interaction Research Center (VOICE, MRC), Kyungpook National University, Daegu, South Korea.
Yu Jin KownCollege of Pharmacy, Research Institute of Pharmaceutical Sciences, Vessel-Organ Interaction Research Center (VOICE, MRC), Kyungpook National University, Daegu, South Korea.
Won-Young ChoiCollege of Pharmacy, Research Institute of Pharmaceutical Sciences, Vessel-Organ Interaction Research Center (VOICE, MRC), Kyungpook National University, Daegu, South Korea.
Ho Jae HanCollege of Veterinary Medicine, Seoul National University, Seoul, South Korea.
Kyung Min BaekCollege of Oriental Medicine, Daegu Haany University, Daegu, South Korea.
Woochul ChangCollege of Education, Pusan National University, Busan, South Korea.
Joong Sun KimCollege of Veterinary Medicine, Chonnam National University, Gwangju, South Korea.
Kyung Seob LimFuturistic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, South Korea.
Seung Pil YunCollege of Medicine, Gyeongsang National University, Jinju, South Korea.
Min Young LeeCollege of Pharmacy, Research Institute of Pharmaceutical Sciences, Vessel-Organ Interaction Research Center (VOICE, MRC), Kyungpook National University, Daegu, South Korea. vetmedic@knu.ac.kr.ORCID http://orcid.org/0000-0003-0067-2803
Kyungpook National University · KRChonnam National University · KRDaegu Haany University · KRGyeongsang National University · KRKorea Research Institute of Bioscience and Biotechnology · KRPusan National University · KRSeoul National University · KR

Funding

National Research Foundation of Korea 2020R1A5A2017323National Research Foundation of Korea 2021R1F1A1047379
6 · The paper itself

Abstract

Human embryonic stem cells (hESCs) have unique characteristics, such as self-renewal and pluripotency, which are distinct from those of other cell types. These characteristics of hESCs are tightly regulated by complex signaling mechanisms. In this study, we demonstrate that yes-associated protein (YAP) functions in an hESC-specific manner to maintain self-renewal and survival in hESCs. hESCs were highly sensitive to YAP downregulation to promote cell survival. Interestingly, hESCs displayed dynamic changes in YAP expression in response to YAP downregulation. YAP was critical for the maintenance of self-renewal. Additionally, the function of YAP in maintenance of self-renewal and cell survival was hESC-specific. Doxycycline upregulated YAP in hESCs and attenuated the decreased cell survival induced by YAP downregulation. However, decreased expression of self-renewal markers triggered by YAP downregulation and neural/cardiac differentiation were affected by doxycycline treatment. Collectively, the results reveal the mechanism underlying the role of YAP and the novel function of doxycycline in hESCs.

Indexed as

Human Embryonic Stem CellsCell DifferentiationDoxycyclineHumansSignal TransductionYAP-Signaling ProteinsDoxycyclineYAP-Signaling ProteinsDoxycyclineHuman embryonic stem cellsSelf-renewalSurvivalYes-associated protein

Identifiers

PMID36219276
PMCPMC11802944
OpenAlexW4304190469

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.