ArticlePlant cell reports2022
Ectopic expression of WOX5 promotes cytokinin signaling and de novo shoot regeneration.
Article in Plant cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Newly found functions and regulatory networks of WOX family: what else does the WOX say?Planta · 2026Review
- Cross-Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana-Derived WUS2 Developmental Regulator.Plant biotechnology journal · 2026Article
- Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferation.Nature communications · 2026Article
- Efficient and cost-effective shoot regeneration in Aguaymanto (Physalis peruviana L.) using meta-Topolin and 6-benzylaminopurine combinations.Journal, genetic engineering & biotechnology · 2025Article
- MONOPTEROS isoform MP11ir plays a role during somatic embryogenesis in Arabidopsis thaliana.Plant physiology · 2025Article
- Rational design of induced regeneration via somatic embryogenesis in the absence of exogenous phytohormones.The Plant cell · 2025Article
- From root to embryogenic transition: WOX5 reprograms plant somatic cells via auxin-mediated pathways.BMC plant biology · 2025Article
- Root regeneration under miRNA control.Nature plants · 2025Article
- Comprehensive analysis and identification of the WOX gene family in Schima superba and the key gene SsuWOX1 for enhancing callus regeneration capacity.BMC plant biology · 2025Article
- A highly efficient soybean transformation system using GRF3-GIF1 chimeric protein.Journal of integrative plant biology · 2025Article
- The Role of Stem Cell Polyphenols in Wound Healing: A Narrative Review.World journal of plastic surgery · 2025Review
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- An inducible CRISPR activation tool for accelerating plant regeneration.Plant communications · 2024Article
- MicroRNA396 negatively regulates shoot regeneration in tomato.Horticulture research · 2024Article
- Plant Growth Regulation in Cell and Tissue Culture In Vitro.Plants (Basel, Switzerland) · 2024Review
- Functional Modules in the Meristems: "Tinkering" in Action.Plants (Basel, Switzerland) · 2023Review
- Cytokinins - regulators ofFrontiers in plant science · 2023Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
key messageWOX5 has a potential in activating cytokinin signaling and shoot regeneration, in addition to its role in pluripotency acquisition. Thus, overexpression of WOX5 maximizes plant regeneration capacity during tissue culture. In vitro plant regeneration involves two steps: callus formation and de novo shoot organogenesis. The WUSCHEL-RELATED HOMEOBOX 5 (WOX5) homeodomain transcription factor is known to be mainly expressed during incubation on callus-inducing medium (CIM) and involved in pluripotency acquisition in callus, but whether WOX5 also affects de novo shoot regeneration on cytokinin-rich shoot-inducing medium (SIM) remains unknown. Based on the recent finding that WOX5 promotes cytokinin signaling, we hypothesized that ectopic expression of WOX5 beyond CIM would further enhance overall plant regeneration capacity, because intense cytokinin signaling is particularly required for shoot regeneration on SIM. Here, we found that overexpression of the WOX5 gene on SIM drastically promoted de novo shoot regeneration from callus with the repression of type-A ARABIDOPSIS RESPONSE REGULATOR (ARR) genes, negative regulators of cytokinin signaling. The enhanced shoot regeneration phenotypes were indeed dependent on cytokinin signaling, which were partially suppressed in the progeny derived from crossing WOX5-overexpressing plants with cytokinin-insensitive 35S:ARR7 plants. The function of WOX5 in enhancing cytokinin-dependent shoot regeneration is evolutionarily conserved, as conditional overexpression of OsWOX5 on SIM profoundly enhanced shoot regeneration in rice callus. Overall, our results provide the technical advance that maximizes in vitro plant regeneration by constitutively expressing WOX5, which unequivocally promotes both callus pluripotency and de novo shoot regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.