Evidence map›Paper›PMID 36219067›Full record

ArticleParasite (Paris, France)2022

Molecular characterization of Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis in laboratory rodents in China.

Nanhao Wang, Ke Wang, Yufeng Liu, Xiaotian Zhang, Jinfeng Zhao, Sumei Zhang, Longxian Zhang

Abstract read
In one paragraph

Article in Parasite (Paris, France), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  3. Occurrence and Molecular Characterization ofPathogens (Basel, Switzerland) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nanhao WangCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, PR China - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, Henan, PR China - Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, Zhengzhou, Henan, PR China.ORCID 0000-0002-8269-7010
Ke WangCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, PR China - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, Henan, PR China - Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, Zhengzhou, Henan, PR China.
Yufeng LiuCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, PR China - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, Henan, PR China - Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, Zhengzhou, Henan, PR China.
Xiaotian ZhangCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, PR China - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, Henan, PR China - Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, Zhengzhou, Henan, PR China.
Jinfeng ZhaoCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, PR China.
Sumei ZhangCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, PR China - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, Henan, PR China.
Longxian ZhangCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, PR China - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, Henan, PR China.ORCID 0000-0002-5706-131X

Funding

National Natural Science Foundation of China 32172882, U1904203the Key Program of the National Natural Science Foundation of China 31330079
6 · The paper itself

Abstract

Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis are significant zoonotic intestinal pathogens that can cause gastrointestinal symptoms such as diarrhea and induce a host immune response. A total of 1237 fecal samples were collected from laboratory rodents (rats, mice and guinea pigs) from four different locations in China to investigate the infection rates and molecular characterization of these pathogens on experimental animals. Genomic DNA was extracted from each sample, and PCR amplifications were done. Overall, the Cryptosporidium spp. infection rate was 3.8% (47/1237). Four known Cryptosporidium species were identified, namely C. parvum, C. muris, C. tyzzeri and C. homai, the three former being zoonotic species. The overall E. bieneusi infection rate was 3.0% (37/1237). Seven known E. bieneusi genotypes, namely S7, BEB6, J, Henan-IV, CHG10, D and WL6, were detected by sequence analysis. Among these, genotypes D, Henan-IV and CHG10 have a high zoonotic risk. Giardia duodenalis was not detected at any of the three loci (SSU rRNA, bg and gdh) after PCR amplification. This study provides basic data for these pathogens in laboratory rodents in China and lays the foundation for their prevention and control in laboratory animals.

Indexed as

CryptosporidiosisCryptosporidiumEnterocytozoonGiardia lambliaGiardiasisMicrosporidiosisAnimalsChinaFecesGenotypeGuinea PigsMiceRatsRodentiaCryptosporidium sppEnterocytozoon bieneusiGiardia duodenalisLaboratory rodentsMolecular characterization

Identifiers

PMID36219067
PMCPMC9552759

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.