ArticleeLife2022
EZ Clear for simple, rapid, and robust mouse whole organ clearing.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed.
- Topological Tracks Patterned via 3D Printing Vascularize Murine Organ-Scale Constructs.Cell biomaterials · 2026Article
- Spectroscopic correlation tomography (SpCT) for visualization of spatial correlations in volumetric OCT scans.Biomedical optics express · 2026Article
- Article
- A human corticospinal organoid-slice connectoid model informs enhancer strategies for post-injury axon regrowth.Cell reports · 2026Article
- Spatially controlled Islet delivery into a bioengineered vascular Niche improves subcutaneous transplantation.Biomedical microdevices · 2026Article
- Advanced tissue clearing and three-dimensional imaging approaches to visualize neural innervation in the rat knee joints.Npj imaging · 2026Article
- Resilience of the bone marrow vascular architecture to aging and hematological stress.Blood vessels, thrombosis & hemostasis · 2026Article
- Frequency-dependent cerebellar circuits independently gate social vocalizations and movement.bioRxiv : the preprint server for biology · 2026Article
- 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D.Cell reports methods · 2026Article
- Comparative analysis of clearing methods for 3D imaging of the vasculature in mineralized mouse tissues.iScience · 2026Article
- Sympathetic nerve-fibroblast crosstalk drives nerve injury, fibroblast activation, and matrix remodeling in pancreatic cancer.JCI insight · 2026Article
- Distinct Endothelial Phenotype Associates with Macrophage-Enriched Microenvironments in Triple-Negative Breast Cancer.Research square · 2026Article
- Article
- Spatially Resolved Mapping of Monoacylglycerol Lipase Activity in the Brain.ACS chemical neuroscience · 2025Article
- Synaptic Gpr85 Influences Cerebellar-Granule-Cell Electrical Properties and Light-Induced Behavior in Zebrafish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Article
- Depth-variant deconvolution applied to widefield microscopy for rapid large-volume tissue imaging.Communications biology · 2025Article
- Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue.Nature biotechnology · 2025Article
- Single-nucleus profiling highlights the all-brain echinoderm nervous system.Science advances · 2025Article
- Emergence of endothelial subtypes and role of cell cycle control in arterial-venous specification during embryonic vascular development.Cell reports · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Tissue clearing for whole organ cell profiling has revolutionized biology and imaging for exploration of organs in three-dimensional space without compromising tissue architecture. But complicated, laborious procedures, or expensive equipment, as well as the use of hazardous, organic solvents prevent the widespread adoption of these methods. Here, we report a simple and rapid tissue clearing method, EZ Clear, that can clear whole adult mouse organs in 48 hr in just three simple steps. Samples stay at room temperature and remain hydrated throughout the clearing process, preserving endogenous and synthetic fluorescence, without altering sample size. After wholemount clearing and imaging, samples processed with EZ Clear can be subjected to downstream applications, such as tissue embedding and cryosectioning followed by standard histology or immunofluorescent staining without loss of fluorescence signal from endogenous or synthetic reporters. Furthermore, we demonstrate that wholemount adult mouse brains processed with EZ Clear can be successfully immunolabeled for fluorescent imaging while still retaining signal from endogenous fluorescent reporters. Overall, the simplicity, speed, and flexibility of EZ Clear make it easy to adapt and implement in diverse imaging modalities in biomedical research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.