Evidence map›Paper›PMID 36214976›Full record

ArticleMolecular biotechnology2023

Paired Box 5-Induced LINC00467 Upregulation Promotes the Progression of Laryngeal Squamous Cell Cancer by Triggering the MicroRNA-4735-3p/TNF Alpha-Induced Protein 3 Pathway.

Yuan Li, Yong Wu, Lili Dai, Honglin Wu, Chaohui Chen, Jiayu Ni, Enli Jin, Xuehua Zhou

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Yuan Li *Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China.
Yong Wu *Department of Otorhinolaryngology, The First People's Hospital of Tongxiang, Jiaxing, China.
Lili DaiDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China.
Honglin WuDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China.
Chaohui ChenDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China.
Jiayu NiDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China.
Enli JinDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China.
Xuehua ZhouDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang Province, China. xuehuazhou22@163.com.ORCID http://orcid.org/0000-0003-3259-3392
Affiliated Hospital of Hangzhou Normal University · CNFirst Hospital of Jiaxing · CNHarbin Medical University · CN

Funding

Development project of science and Technology of Hangzhou 20110833B09Medical and health research project of Zhejiang Province 2017RC011National Natural Science Foundation of Zhejiang province, China Y2090486National Natural Science Foundation of Zhejiang province, China Y2100578
6 · The paper itself

Abstract

LINC00467 was reported as an oncogenic gene in different types of human cancers. In this study, we investigated the molecular mechanisms of LINC00467 in the tumorigenesis of laryngeal squamous cell cancer (LSCC). RT-qPCR was utilized to detect the mRNA expression of genes, and western blot assay was used to determine the protein levels of TNF alpha-induced protein 3 (TNFAIP3). The cell viability was detected by CCK-8 assay. Transwell assays were conducted to determine the cell migration and invasion of LSCC cells, and the cell cycle was assessed by flow cytometry. The association between paired box 5 (PAX5), LINC00467, miR-4735-3p, and TNFAIP3 was verified using ChIP, RNA pull-down, or luciferase reporter assays. In our study, we found that LINC00467 was upregulated in LSCC, and knockdown of LINC00467 suppressed cell viability and metastasis of LSCC. Besides, LINC00467 transcription could be activated by PAX5 in LSCC. Furthermore, LINC00467 acted as competitive endogenous RNA (ceRNA) for miR-4735-3p to accelerate LSCC progression. In the meantime, TNFAIP3 was identified as a downstream gene of miR-4735-3p. Finally, TNFAIP3 overexpression could overturn the effects of miR-4735-3p mimic on LSCC cellular activities. In conclusion, our results demonstrated that PAX5-induced LINC00467 facilitated LSCC progression by inhibiting miR-4735-3p to increase TNFAIP3 expression.

Indexed as

Carcinoma, Squamous CellHead and Neck NeoplasmsLaryngeal NeoplasmsMicroRNAsCell Line, TumorCell ProliferationGene Expression Regulation, NeoplasticHumansSquamous Cell Carcinoma of Head and NeckTumor Necrosis Factor-alphaUp-RegulationMicroRNAsTumor Necrosis Factor-alphaLaryngeal squamous cell cancer cellsLINC00467miR-4735-3pPAX5TNFAIP3

Identifiers

PMID36214976
OpenAlexW4304116280

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.