ArticleFrontiers in veterinary science2022
Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals.
Article in Frontiers in veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 22 citations in OpenAlex.
- Early Detection of Porcine Reproductive and Respiratory Syndrome Virus Outbreak: Combination of Methods.Veterinary sciences · 2025Article
- Characterizing dynamics of PRRSV RNA positivity over time in sows after live virus exposure and associated risk factors based on TOSc samples.BMC veterinary research · 2025Article
- Combining Load-Close-Homogenize with Testing, Removal, and Rollover Strategies to Repopulate PRRSV Elimination Breeding Herds Using PRRSV-Positive Weaned Gilts.Veterinary sciences · 2025Article
- Optimizing PRRSV Detection: The Impact of Sample Processing and Testing Strategies on Tongue Tips.Pathogens (Basel, Switzerland) · 2025Article
- Assessment of Diagnostic Value of Post Mortem Tongue Tip Fluids for Disease Detection in Growing Pigs.Animals : an open access journal from MDPI · 2025Article
- Evaluating postmortem tongue fluids as a tool for monitoring PRRSV and IAV in the post-wean phases of swine production.Porcine health management · 2025Article
- Impact of weaning procedures on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) circulation in the nursery section.BMC veterinary research · 2025Article
- PRRSV RNA Detection and Predictive Values Between Different Sow and Neonatal Litter Sample Types.Veterinary sciences · 2025Article
- Effect of Time and Temperature on the Detection of PRRSV RNA and Endogenous Internal Sample Control in Porcine Tongue Fluids.Veterinary sciences · 2025Article
- Optimizing Tongue Fluid Sampling and Testing Protocols for Enhanced PRRSV Isolation from Perinatal Swine Mortalities.Viruses · 2025Article
- Evaluating stillborn and litter size as indicators of PRRSV detection in live piglets and the use of stillborn tongue fluids as risk-based samples for PRRSV monitoring.Frontiers in veterinary science · 2025Article
- Oropharyngeal swab sampling for PRRSV detection in large-scale pig farms: a convenient and reliable method for mass sampling.Porcine health management · 2024Article
- Characterizing best practices for tonsil-oral-scrubbing (TOSc) collection for PRRSV RNA detection in sows.Porcine health management · 2024Article
- Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection.Pathogens (Basel, Switzerland) · 2024Article
- Detection of PRRSV-1 in tongue fluids under experimental and field conditions and comparison of different sampling material for PRRSV sow herd monitoring.Porcine health management · 2024Article
- Early Detection of PRRSV Outbreaks in Breeding Herds by Monitoring Productivity and Electronic Sow Feed Data Using Univariate and Multivariate Statistical Process Control Methods.Transboundary and emerging diseases · 2024Article
- Comparison of tonsil-oral-scrubbing with serum, oral fluid, and tonsil scraping to detect PRRSV RNA in sows over time following live virus inoculation.Frontiers in veterinary science · 2024Article
- Alternative Samples for Porcine Reproductive and Respiratory Syndrome Surveillance in an Endemic PRRSV-1-Infected Breeding Herd: A Descriptive Study.Veterinary sciences · 2023Article
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. Tongue tips and serum samples were collected from three PRRSV-positive breeding herd farms (farms A, B, and C) of three different age groups: newborns (<24 h), processing (2 to 7 days of age), and weaning (18 to 22 days of age). Additionally, processing fluids and family oral fluids were collected from 2-7 days of age and weaning age, respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all age groups (100 and 95%, respectively). In addition, PRRSV RNA was detected in pooled serum samples (42 and 27%), processing fluids (100 and 50%), and family oral fluids (11 and 22%). Interestingly, the average Ct value from tongue tips was numerically lower than the average Ct value from serum samples in the newborn age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected in tongue tips when pooled serum samples from the same age group tested PRRSV RNA-negative. Taken together, these results demonstrate the potential value of tongue tips for PRRSV monitoring and surveillance.
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