ReviewTranslational animal science2022
Unraveling the pros and cons of various in vitro methodologies for ruminant nutrition: a review.
Review in Translational animal science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Nutritional Strategies for Methane, Nitrogen, and Phosphorus Mitigation in Ruminants: Mechanisms, Applications, and Regional Adaptations.Microorganisms · 2026Review
- Ruminal biohydrogenation and microbial lipids in dairy cows supplemented with fractionated palm oil or Hermetia illucens oil.Journal of animal science · 2026Article
- Development of ovine hepatic organoids: a powerfulFrontiers in veterinary science · 2026Article
- Application of Artificial Gastrointestinal Tract Models in Veterinary Medicine.Animals : an open access journal from MDPI · 2025Review
- Next-generation non-animal models for inflammatory bowel disease:NAM journal · 2025Review
- Effect of best bet methane abatement feed on feed intake, digestibility, live weight change, and methane emission in local Menz breed sheep in Ethiopia.Frontiers in veterinary science · 2025Article
- Development and application of a multi-step porcine in vitro system to evaluate feedstuffs and feed additives for their efficacy in nutrient digestion, digesta characteristics, and intestinal immune responses.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Evaluation of ruminal methane and ammonia formation and microbiota composition as affected by supplements based on mixtures of tannins and essential oils using Rusitec.Journal of animal science and biotechnology · 2024Article
- Trace mineral sources influence in vitro fermentation characteristics and trace mineral solubility.Journal of animal science · 2024Article
- Ruminal inocula with distinct fermentation profiles differentially affect theFrontiers in veterinary science · 2024Article
- Effect of Chitosan on Ruminal Fermentation and Microbial Communities, Methane Emissions, and Productive Performance of Dairy Cattle.Animals : an open access journal from MDPI · 2023Article
- Forages and Pastures Symposium: revisiting mechanisms, methods, and models for altering forage cell wall utilization for ruminants.Journal of animal science · 2023Article
- Forages and pastures symposium: an update on in vitro and in situ experimental techniques for approximation of ruminal fiber degradation.Journal of animal science · 2023Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To decrease the time and cost of experiments as well as the use of animals in nutrition research, in vitro methodologies have become more commonplace in the field of ruminant nutrition. Therefore, the objectives of this review are 1) to describe the development of different in vitro methodologies, 2) to discuss the application, utilization, and advantages of in vitro methodologies, 3) to discuss shortcomings of in vitro methodologies, and 4) to describe the potential developments that may be able to improve in vitro methods. Having been used for decades, some in vitro methodologies such as pure, batch, and continuous cultures have been very well documented and utilized to investigate a wide array of different aspects of nutrition, including the effects of different dietary compositions, individual fermentation end products, and impacts on the microbiome of the rumen. However, both batch and pure cultures can result in a build-up of end products that may inhibit fermentation, as they culture ruminal contents or defined strains of bacteria, respectfully. Continuous culture; however, allows for the removal of end products but, similar to pure and batch cultures, is applicable only to ruminal fermentation and cannot provide information regarding intestinal digestion and bioavailability. This information for in vitro can only be provided using an assay designed for total tract digestibility, which is the three-step procedure (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.