Evidence map›Paper›PMID 36212714›Full record

ReviewBioMed research international2022

A Comprehensive Review of Performance of Next-Generation Sequencing Platforms.

Muhammad Tariq Pervez, Mirza Jawad Ul Hasnain, Syed Hassan Abbas, Mahmoud F Moustafa, Naeem Aslam, Syed Shah Muhammad Shah

RetractedAbstract readReviewRetracted Publication
In one paragraph

Review in BioMed research international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. The Evolution of Next-Generation Sequencing Technologies.Methods in molecular biology (Clifton, N.J.) · 2025
    Review
  10. Review
  11. Article
  12. Article
  13. Genotypic and phenotypic spectrum of maple syrup urine disease in Zhejiang of China.QJM : monthly journal of the Association of Physicians · 2024
    Article
  14. Review
  15. Article
  16. Utility Analyses of AVITI Sequencing Chemistry.bioRxiv : the preprint server for biology · 2024
    Article
  17. Exome and genome sequencing in a heterogeneous population of patients with rare disease: Identifying predictors of a diagnosis.Genetics in medicine : official journal of the American College of Medical Genetics · 2024
    Article
  18. Review
  19. Update on the proposed minimal standards for the use of genome data for the taxonomy of prokaryotes.International journal of systematic and evolutionary microbiology · 2024
    Article
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Muhammad Tariq PervezDepartment of Bioinformatics and Computational Biology, Virtual University of Pakistan, Pakistan.ORCID https://orcid.org/0000-0002-7946-5980
Mirza Jawad Ul HasnainDepartment of Bioinformatics and Computational Biology, Virtual University of Pakistan, Pakistan.ORCID https://orcid.org/0000-0002-4949-8060
Syed Hassan AbbasDepartment of Bioinformatics and Computational Biology, Virtual University of Pakistan, Pakistan.
Mahmoud F MoustafaDepartment of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
Naeem AslamDepartment of Computer Science, NFCIET, Khanewal Road, Multan, Pakistan.
Syed Shah Muhammad ShahDepartment of Computer Science, Virtual University of Pakistan, Pakistan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Next-generation sequencing methods have been developed and proposed to investigate any query in genomics or clinical activity involving DNA. Technical advancement in these sequencing methods has enhanced sequencing volume to several billion nucleotides within a very short time and low cost. During the last few years, the usage of the latest DNA sequencing platforms in a large number of research projects helped to improve the sequencing methods and technologies, thus enabling a wide variety of research/review publications and applications of sequencing technologies. Objective: The proposed study is aimed at highlighting the most fast and accurate NGS instruments developed by various companies by comparing output per hour, quality of the reads, maximum read length, reads per run, and their applications in various domains. This will help research institutions and biological/clinical laboratories to choose the sequencing instrument best suited to their environment. The end users will have a general overview about the history of the sequencing technologies, latest developments, and improvements made in the sequencing technologies till now. Results: The proposed study, based on previous studies and manufacturers' descriptions, highlighted that in terms of output per hour, Nanopore PromethION outperformed all sequencers. BGI was on the second position, and Illumina was on the third position. Conclusion: The proposed study investigated various sequencing instruments and highlighted that, overall, Nanopore PromethION is the fastest sequencing approach. BGI and Nanopore can beat Illumina, which is currently the most popular sequencing company. With respect to quality, Ion Torrent NGS instruments are on the top of the list, Illumina is on the second position, and BGI DNB is on the third position. Secondly, memory- and time-saving algorithms and databases need to be developed to analyze data produced by the 3

Indexed as

GenomicsHigh-Throughput Nucleotide SequencingAlgorithmsHumansNucleotidesSequence Analysis, DNANucleotides

Identifiers

PMID36212714
PMCPMC9537002

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.