Evidence map›Paper›PMID 36212290›Full record

ArticleFrontiers in plant science2022

Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf.

Masaki Odahara, Yoko Horii, Jun Itami, Kenta Watanabe, Keiji Numata

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells.International journal of molecular sciences · 2023
    Review
  5. Article
  6. Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023
    Review
  7. Plastid Transformation: New Challenges in the Circular Economy Era.International journal of molecular sciences · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Masaki OdaharaBiomacromolecule Research Team, RIKEN Center for Sustainable Resource Science, Wako, Japan.
Yoko HoriiBiomacromolecule Research Team, RIKEN Center for Sustainable Resource Science, Wako, Japan.
Jun ItamiBiomacromolecule Research Team, RIKEN Center for Sustainable Resource Science, Wako, Japan.
Kenta WatanabeBiomacromolecule Research Team, RIKEN Center for Sustainable Resource Science, Wako, Japan.
Keiji NumataBiomacromolecule Research Team, RIKEN Center for Sustainable Resource Science, Wako, Japan.
RIKEN Center for Sustainable Resource Science · JPKyoto University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In plant engineering, plastid transformation is more advantageous than nuclear transformation because it results in high levels of protein expression from multiple genome copies per cell and is unaffected by gene silencing. The common plastid transformation methods are biolistic bombardment that requires special instruments and PEG-mediated transformation that is only applicable to protoplast cells. Here, we aimed to establish a new plastid transformation method in tobacco, rice, and kenaf using a biocompatible fusion peptide as a carrier to deliver DNA into plastids. We used a fusion peptide, KH-AtOEP34, comprising a polycationic DNA-binding peptide (KH) and a plastid-targeting peptide (AtOEP34) to successfully deliver and integrate construct DNA into plastid DNA (ptDNA)

Indexed as

functional peptidegene deliverygene targetingkenafplastid transformationricetobacco

Identifiers

PMID36212290
PMCPMC9539840
OpenAlexW4296840686

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.