Evidence map›Paper›PMID 36211215›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2022

Coincidence of v-raf murine sarcoma viral oncogene homolog B mutation (V595E) with phosphorylated v-raf murine sarcoma viral oncogene homolog B in urothelial carcinoma in dogs.

Hirofumi Yamasaki, Yosuke Uematsu, Yuhei Hayashi, Masao Yamashita, Meina Tei, Kazuyuki Uchida, Kenichiro Ono, Hidehiro Hirao

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 78% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Antitumor effects of CEP32496 on urothelial carcinoma with BRAF mutation (V595E) in dogs.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Hirofumi YamasakiJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Yosuke UematsuJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Yuhei HayashiJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Masao YamashitaJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Meina TeiJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Kazuyuki UchidaJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Kenichiro OnoJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Hidehiro HiraoJapan Animal Referral Medical Center, Kawasaki, Japan (Yamasaki, Hayashi, Yamashita, Tei, Ono, Hirao); Canine-Lab, Tokyo, Japan (Uematsu); Department of Veterinary Pathology, The University of Tokyo (Uchida).
Canon (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expression of phosphorylated v-raf murine sarcoma viral oncogene homolog B (pBRAF) and phosphorylated extracellular signal-regulated kinase1/2 (pERK1/2) were investigated in urothelial carcinoma (UC) in dogs with or without the BRAF gene mutation (V595E). Among the 10 cases of UC with V595E (-), cytoplasmic immunoreactivity against pBRAF of neoplastic cells was reported in 8, with 7 displaying moderate reactivity and 1 displaying intense reactivity. Nuclear immunoreactivity against pBRAF was detected in 5 cases; however, these reactivities were non-specific, due to pBRAF being limited in the cytoplasm. In addition, positive cytoplasmic immunoreactivity against pERK1/2 of neoplastic cells was detected in 7 cases and nuclear immunoreactivity against ERK1/2 was detected in 6 cases. Among the 13 cases of UC with V595E (+), cytoplasmic immunoreactivity against pBRAF of neoplastic cells was detected in all 13 cases and nuclear immunoreactivity against pBRAF was detected in 10 cases; however, the nuclear immunoreactivity was non-specific. Cytoplasmic immunoreactivity against pERK1/2 of neoplastic cells was detected in all 13 cases and nuclear immunoreactivity against pERK1/2 was also detected in all cases. As nuclear pERK1/2 indicates a progressive signaling process in the mitogen-activated protein kinase pathway, V595E (+) UC might be in its growing stage. Probable phosphorylated sites of pBRAF at Thr598/Ser601, detected in this study, are major and essential sites of the upstream rat sarcoma viral oncogene homolog (RAS) signaling pathway. In human cancers, the

Indexed as

Carcinoma, Transitional CellDog DiseasesUrinary Bladder NeoplasmsAnimalsDogsHumansMiceMutationOncogenesProto-Oncogene Proteins B-rafProto-Oncogene Proteins p21(ras)Proto-Oncogene Proteins B-rafProto-Oncogene Proteins p21(ras)

Identifiers

PMID36211215
PMCPMC9536355
OpenAlexW4304117130

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.