Evidence map›Paper›PMID 36208306›Full record

ArticlePlant cell reports2022

A single QTL harboring multiple genetic variations leads to complicated phenotypic segregation in apple flesh firmness and crispness.

Xianglong Yang, Bei Wu, Jing Liu, Zhongyan Zhang, Xuan Wang, Haie Zhang, Xuejun Ren, Xi Zhang, Yi Wang, Ting Wu and 3 more

Abstract read
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In one paragraph

Article in Plant cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
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  4. Genetic variations inMolecular breeding : new strategies in plant improvement · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Xianglong YangCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Bei WuCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Jing LiuCollege of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Zhongyan ZhangCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Xuan WangCollege of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Haie ZhangCollege of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Xuejun RenTesting and Analysis Center, Hebei Normal University of Science and Technology, Qinhuangdao, 066600, China.
Xi ZhangCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Yi WangCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Ting WuCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Xuefeng XuCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Zhenhai HanCollege of Horticulture, China Agricultural University, Beijing, 100193, China.
Xinzhong ZhangCollege of Horticulture, China Agricultural University, Beijing, 100193, China. zhangxinzhong999@126.com.ORCID http://orcid.org/0000-0002-7038-8667
China Agricultural University · CNHebei Normal University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messageWithin a QTL, the genetic recombination and interactions among five and two functional variations at MdbHLH25 and MdWDR5A caused much complicated phenotype segregation in apple FFR and FCR. The storability of climacteric fruit like apple is a quantitative trait. We previously identified 62 quantitative trait loci (QTLs) associating flesh firmness retainability (FFR) and flesh crispness retainability (FCR), but only a few functional genetic variations were identified and validated. The genetic variation network controlling fruit storability is far to be understood and diagnostic markers are needed for molecular breeding. We previously identified overlapped QTLs F16.1/H16.2 for FFR and FCR using an F1 population derived from 'Zisai Pearl' × 'Red Fuji'. In this study, five and two single-nucleotide polymorphisms (SNPs) were identified on the candidate genes MdbHLH25 and MdWDR5A within the QTL region. The SNP1 A allele at MdbHLH25 promoter reduced the expression and SNP2 T allele and/or SNP4/5 GT alleles at the exons attenuated the function of MdbHLH25 by downregulating the expression of the target genes MdACS1, which in turn led to a reduction in ethylene production and maintenance of higher flesh crispness. The SNPs did not alter the protein-protein interaction between MdbHLH25 and MdWDR5A. The joint effect of SNP genotype combinations by the SNPs on MdbHLH25 (SNP1, SNP2, and SNP4) and MdWDR5A (SNPi and SNPii) led to a much broad spectrum of phenotypic segregation in FFR and FCR. Together, the dissection of these genetic variations contributes to understanding the complicated effects of a QTL and provides good potential for marker development in molecular breeding.

Indexed as

MalusQuantitative Trait LociChromosome MappingPhenotypePolymorphism, Single NucleotideAppleFlesh firmness and crispnessGenetic variationQTL

Identifiers

PMID36208306
OpenAlexW4303648655

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.