ArticlePlant biotechnology journal2023
MPK6-mediated HY5 phosphorylation regulates light-induced anthocyanin accumulation in apple fruit.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 82 citations in OpenAlex.
- Multifaceted mechanisms underlying species- and cultivar-associated differential anthocyanin accumulation in fruits.Horticulture research · 2026Article
- Molecular Mechanisms and Regulation of Anthocyanin Biosynthesis in Rosaceae Fruits: A Review.Food science & nutrition · 2026Review
- Light Regulation of Anthocyanin Biosynthesis in Plants: From Mechanisms to Applications.Plant, cell & environment · 2026Review
- Dual regulation of anthocyanins and proanthocyanidins by PpBL and its linkage to acidity in peach.Molecular horticulture · 2026Article
- The VvBZR1-VvHY5 Module Integrates Brassinosteroid and Light Signaling to Regulate Anthocyanin Biosynthesis in Grape.Plant, cell & environment · 2026Article
- Plant Flavonoids: Biosynthesis, Regulation, and Roles in Biotic and Abiotic Stresses.Plant, cell & environment · 2026Review
- Anthocyanin accumulation in fruit crops: a multi-layered regulatory network integrating molecular, epigenetic, hormonal and environmental regulation.Molecular biology reports · 2026Review
- Anthocyanin-Driven Dark Phenotypes in Stress Adaptation.Plants (Basel, Switzerland) · 2026Review
- LsMAPK6 Phosphorylates the LsCO Protein to Enhance Its Stability and Transcriptional Activity, Promoting Floral Transition Upon High Temperatures in Lettuce.Plant biotechnology journal · 2026Article
- FvMAPK6-Mediated FvMYB44s/FvSWEET1 Dual-Layer Regulation Modulates Sugar Accumulation in Strawberry Fruit, With FvSPS3 Enabling Quality-Yield Balance.Plant biotechnology journal · 2026Article
- Integration of ATAC-seq and RNA-seq profiling reveals an epigenetic regulator of anthocyanin synthesis in Prunus mume.BMC plant biology · 2026Article
- PtrbZIP12 improves drought resistance inHorticulture research · 2026Article
- Genome Assembly Revealed MdZAT5 Coordinates Anthocyanin Biosynthesis in Apple Fruit Peel and Flesh by Interacting With MdHY5.Plant biotechnology journal · 2026Article
- Protein post-translational modifications: key switches coordinating fruit ripening regulatory networks.Horticulture research · 2026Article
- MAPK regulates secondary metabolism and abiotic stress in horticultural and medicinal plants.Horticulture research · 2026Article
- Molecular insights into the regulation of flavonoid biosynthesis in fruits.Horticulture research · 2026Article
- The PavDREB1E-PavD8-PavHY5 module integrates light and gibberellin signals to regulate sweet cherry anthocyanin biosynthesis.Plant physiology · 2025Article
- BnaC04.bZIP16 can be phosphorylated and inhibited by BnaA06.SnRK2 and negatively regulates the accumulation of fatty acids in Brassica napus.The Plant journal : for cell and molecular biology · 2025Article
- Review
- Identification and characterization of DFR gene family and cloning of candidate genes for anthocyanin biosynthesis in pepper (Capsicum annuum L.).BMC plant biology · 2025Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light is known to regulate anthocyanin pigment biosynthesis in plants on several levels, but the significance of protein phosphorylation in light-induced anthocyanin accumulation needs further investigation. In this study, we investigated the dynamics of the apple fruit phosphoproteome in response to light, using high-performance liquid chromatography-tandem mass spectrometry analysis. Among the differentially phosphorylated proteins, the bZIP (basic leucine zipper) transcription factor, HY5, which has been identified as an anthocyanin regulator, was rapidly activated by light treatment of the fruit. We hypothesized that phosphorylated MdHY5 may play a role in light-induced anthocyanin accumulation of apple fruit. Protein interaction and phosphorylation assays showed that mitogen-activated protein kinase MdMPK6 directly interacted with, and activated, MdHY5 via phosphorylation under light conditions, thereby increasing its stability. Consistent with this finding, the suppression of the mitogen-activated protein kinase genes MdMPK6 or MdHY5 resulted in an inhibition of anthocyanin accumulation, and further showed that light-induced anthocyanin accumulation is dependent on MdMPK6 kinase activity, and is required for maximum MdHY5 activity. Under light conditions, active MdMPK6 phosphorylated MdHY5 leading to accumulation of phospho-MdHY5, which enhanced the binding of MdHY5 to its target anthocyanin related genes in fruit. Our findings reveal an MdMPK6-MdHY5 phosphorylation pathway in light-induced anthocyanin accumulation, providing new insights into the regulation of light-induced anthocyanin biosynthesis in apple fruit at both the transcriptional and post-translational levels.
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