ArticleMolecular cell2022
The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
Article in Molecular cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Biochemical Characterization of LsGajA: A Key Nuclease for Gabija Defense in Lactic Acid Bacteria.Microorganisms · 2026Article
- Synthetically designed anti-defense proteins overcome barriers to bacterial transformation and phage infection.Nature communications · 2026Article
- Structural investigation of QatB and QatC proteins in QatABCD anti-phage defense.Nature communications · 2026Article
- The interplay between ecological networks drives host-plasmid community dynamics.PLoS computational biology · 2026Article
- A developmental condensin I complex assists the Paramecium PiggyMac domesticated transposase during programmed DNA elimination.Nucleic acids research · 2026Article
- An update on Escalating Drug Resistance among Uropathogenic Bacteria: Challenges in Treatment and Scope for Maneuver.Current microbiology · 2026Review
- Supercoiled DNA recognition and cleavage control in topoisomerase VI.Nature communications · 2026Article
- It's not me, it's you: Anti-phage nuclease specificity inside a bacterium.PLoS pathogens · 2026Article
- Article
- Architecture and mechanism of a dual-enzyme retron system in prokaryotic immunity.Nature communications · 2025Article
- The SMC Hinge is a Selective Gate for Obstacle Bypass.Nature communications · 2025Article
- UsingACS synthetic biology · 2025Article
- Structural basis of antiphage defence by an ATPase-associated reverse transcriptase.Nature communications · 2025Article
- Abundant bacterial nucleoid-associated protein H-NS limits plasmid transfer through mechanical modification of DNA.Nucleic acids research · 2025Article
- Structural basis of the RNA-mediated Retron-Eco2 oligomerization.Cell discovery · 2025Article
- Replisomes restrict SMC translocation in vivo.Nature communications · 2025Article
- SMC-like Wadjet system prevents plasmid transfer into Clostridium cellulovorans.Applied microbiology and biotechnology · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.Cell · 2025Article
- Article
4 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
Self versus non-self discrimination is a key element of innate and adaptive immunity across life. In bacteria, CRISPR-Cas and restriction-modification systems recognize non-self nucleic acids through their sequence and their methylation state, respectively. Here, we show that the Wadjet defense system recognizes DNA topology to protect its host against plasmid transformation. By combining cryoelectron microscopy with cross-linking mass spectrometry, we show that Wadjet forms a complex similar to the bacterial condensin complex MukBEF, with a novel nuclease subunit similar to a type II DNA topoisomerase. Wadjet specifically cleaves closed-circular DNA in a reaction requiring ATP hydrolysis by the structural maintenance of chromosome (SMC) ATPase subunit JetC, suggesting that the complex could use DNA loop extrusion to sense its substrate's topology, then specifically activate the nuclease subunit JetD to cleave plasmid DNA. Overall, our data reveal how bacteria have co-opted a DNA maintenance machine to specifically recognize and destroy foreign DNAs through topology sensing.
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