Evidence map›Paper›PMID 36205906›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Cytotoxicity Models in Chromaffin Cells to Evaluate Neuroprotective Compounds.

María F Cano-Abad, Manuela G López

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Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

María F Cano-AbadInstituto Teófilo Hernando. Departamento de Farmacología. Facultad de Medicina. Universidad Autónoma de Madrid and Instituto de investigación Sanitaria Hospital de la Princesa, Madrid, Spain.
Manuela G LópezInstituto Teófilo Hernando. Departamento de Farmacología. Facultad de Medicina. Universidad Autónoma de Madrid and Instituto de investigación Sanitaria Hospital de la Princesa, Madrid, Spain. manuela.garcia@uam.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primary cultures of bovine chromaffin cells are considered a good model to evaluate potential neuroprotective compounds for two major reasons: (i) they share many common features to neurons as they synthesize, store, and release neurotransmitters; they are excitable cells that express voltage-dependent calcium, potassium, and sodium channels; they express different neuronal receptor subtypes; and (ii) they can be easily cultured in high quantities from adult animals; as adult para-neurons, they can be used to reproduce different neurodegenerative-like cytotoxicity models. In this chapter, we describe protocols to mimic calcium overload (veratridine and thapsigargin) and oxidative stress (rotenone plus oligomycin-A and 6-hydroxydopamine) to evaluate potential neuroprotective compounds.

Indexed as

Chromaffin CellsNeuroprotective AgentsAnimalsCalciumCattleCells, CulturedNeurotransmitter AgentsOligomycinsOxidopaminePotassiumRotenoneSodium ChannelsThapsigarginVeratridineCalciumNeuroprotective AgentsNeurotransmitter AgentsOligomycinsOxidopaminePotassiumRotenoneSodium ChannelsThapsigarginVeratridine6-HydroxydopamineCalcium overloadChromaffin cellsCytotoxicityDrug screeningFree radicalsLDHNeuroprotectionVeratridine

Identifiers

PMID36205906

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.