Evidence map›Paper›PMID 36205888›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Visualization of Exo- and Endocytosis Membrane Dynamics with Super-Resolution STED Microscopy.

Chung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo, Ling-Gang Wu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.7field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Chung Yu ChanNational Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. keith.chan@nih.gov.
Sue HanNational Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
Xin WangNational Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
Xiaoli GuoNational Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
Ling-Gang WuNational Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. wul@ninds.nih.gov.
National Institute of Neurological Disorders and Stroke · US

Funding

Synaptic Vesicle ExocytosisZIANS003009 · NINDS · NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE · PI WU, LING GANG · 2009 to 2025
$31.2M
Synaptic Vesicle EndocytosisZIANS003105 · NINDS · NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE · PI WU, LING GANG · 2009 to 2025
$30.9M
Intramural NIH HHS ZIA NS003009Intramural NIH HHS ZIA NS003105
6 · The paper itself

Abstract

Recent advances in stimulated emission depletion (STED) microscopy offer an unparalleled avenue to study membrane dynamics of exo- and endocytosis, such as fusion pore opening, pore expansion, constriction, and closure, as well as the membrane transformation from flat-shaped to round-shaped vesicles in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This method can potentially be applied to study other membrane structure dynamics in other cell model system.

Indexed as

Chromaffin CellsMicroscopyAnimalsCattleCell MembraneEndocytosisSecretory VesiclesEndocytosisExocytosisFusion poreSTED microscopy

Identifiers

PMID36205888
OpenAlexW4303519294

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.