Evidence map›Paper›PMID 36204331›Full record

ArticleCurrent developments in nutrition2022

Ingestion of Indigestible Cacao Proteins Promotes Defecation and Alters the Intestinal Microbiota in Mice.

Jinichiro Koga, Kota Ojiro, Ayumi Yanagida, Takahisa Suto, Hideaki Hiki, Yuki Inoue, Chihiro Sakai, Kohei Nakamoto, Yuta Fujisawa, Ayaka Orihara and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current developments in nutrition, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Jinichiro KogaDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.ORCID https://orcid.org/0000-0003-2106-9894
Kota OjiroDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Ayumi YanagidaDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Takahisa SutoDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Hideaki HikiDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Yuki InoueDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Chihiro SakaiDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Kohei NakamotoDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Yuta FujisawaDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Ayaka OriharaDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Haruka MurakamiDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Shintaro HirasawaDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Kengo NakajimaDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Tomoko SakazawaDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Hisakazu YamaneDepartment of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, Japan.
Teikyo University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: In animals, the health effects of ingested cacao proteins are unknown because the proteins are difficult to extract and purify from cacao beans. Objectives: This study aimed to develop an extraction and purification method for cacao proteins and reveal the effect of ingestion of cacao proteins on defecation and intestinal microbiota in mice. Methods: Three groups of mice were fed a control diet (AIN-93 G), a cacao lignin diet (AIN-93 G containing 1.22% cacao lignin), or a cacao protein and lignin diet (AIN-93 G containing 1.97% cacao proteins and 1.22% cacao lignin) by pair-feeding for 8 d. Feces were collected as 2 bulked samples from days 1 to 4 and days 5 to 8 on each diet. The collected feces were weighed and the intestinal microbiota was analyzed by next-generation sequencing-based 16S rRNA. Results: A new extraction and purification method for cacao proteins has been developed, then found that the proteins are resistant to digestive enzymes. However, the cacao protein powder made by this method contained 34.9% of lignin in addition to 56.4% of proteins. Therefore, to reveal the effect by cacao proteins alone, the fecal weight and intestinal microbiota of mice fed the cacao protein and lignin diet were compared with those of mice fed the cacao lignin diet. The fecal weight of mice fed the cacao protein and lignin diet was significantly greater than of mice fed the cacao lignin diet. The relative abundance of Conclusions: Ingestion of indigestible cacao proteins promoted defecation and altered the intestinal microbiota such as

Indexed as

Anaerotruncuscacao proteinschocolatedefecationintestinal microbiotaLactococcusMucispirillumOscillospiraprebioticsTheobroma cacao L.

Identifiers

PMID36204331
PMCPMC9529219
OpenAlexW4290005531

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.