Evidence map›Paper›PMID 36203076›Full record

ArticleCell biochemistry and biophysics2023

Spectral Phasor Analysis of Nile Red Identifies Membrane Microenvironment Changes in the Presence of Amyloid Peptides.

Bhawantha M Jayawardena, Resmi Menon, Mark R Jones, Christopher E Jones

Abstract read
PubMed Publisher
In one paragraph

Article in Cell biochemistry and biophysics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Bhawantha M JayawardenaSchool of Science, Western Sydney University, Locked Bag 1797, Penrith, 2751, NSW, Australia.
Resmi MenonSchool of Science, Western Sydney University, Locked Bag 1797, Penrith, 2751, NSW, Australia.
Mark R JonesSchool of Science, Western Sydney University, Locked Bag 1797, Penrith, 2751, NSW, Australia.
Christopher E JonesSchool of Science, Western Sydney University, Locked Bag 1797, Penrith, 2751, NSW, Australia. c.jones@westernsydney.edu.au.
Western Sydney University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The interaction of protein and peptide amyloid oligomers with membranes is thought to be one of the mechanisms contributing to cellular toxicity. However, techniques to study these interactions in the complex membrane environment of live cells are lacking. Spectral phasor analysis is a recently developed biophysical technique that can enable visualisation and analysis of membrane-associated fluorescent dyes. When the spectral profile of these dyes changes as a result of changes to the membrane microenvironment, spectral phasor analysis can localise those changes to discrete membrane regions. In this study, we investigated whether spectral phasor analysis could detect changes in the membrane microenvironment of live cells in the presence of fibrillar aggregates of the disease-related Aβ

Indexed as

Membrane FluidityOxazinesAmyloidAmyloid beta-PeptidesCell MembraneFluorescent DyesPeptidesAmyloidAmyloid beta-PeptidesFluorescent Dyesnile redOxazinesPeptidesFluorescenceFunctional amyloidNeurokininSpectral phasor analysis

Identifiers

PMID36203076
OpenAlexW4302362848

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.