ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2022
Single Nuclei Analyses Reveal Transcriptional Profiles and Marker Genes for Diverse Supraspinal Populations.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 21 citations in OpenAlex.
- Chronic activation of corticospinal tract neurons after pyramidotomy injury enhances neither behavioral recovery nor axonal sprouting.Experimental neurology · 2026Article
- A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.Pharmacological reports : PR · 2026Article
- Descending inhibitory rostral ventromedial medulla neurons cause widespread antinociception and contribute to the pain-inhibits-pain phenomenon.Nature communications · 2026Article
- A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types.Cell reports · 2026Article
- Retrograde viral delivery of hyper-IL-6 activates Stat3 in corticospinal tract neurons but causes severe tremors and weight loss in adult mice.bioRxiv : the preprint server for biology · 2025Article
- Sox11 overexpression restores embryonic pro-growth transcription in mature corticospinal tract neurons.Scientific reports · 2025Article
- A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types.Research square · 2025Article
- Sox11 overexpression restores embryonic pro-growth transcription in mature corticospinal tract neurons.bioRxiv : the preprint server for biology · 2025Article
- From thought to action: The organization of spinal projecting neurons.Cell reports · 2025Review
- Projection-TAGs enable multiplex projection tracing and multi-modal profiling of projection neurons.Nature communications · 2025Article
- Projection-TAGs enable multiplex projection tracing and multi-modal profiling of projection neurons.bioRxiv : the preprint server for biology · 2025Article
- Single-Nuclei Sequencing Reveals a Robust Corticospinal Response to Nearby Axotomy But Overall Insensitivity to Spinal Injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- The diversity and plasticity of descending motor pathways rewired after stroke and trauma in rodents.Frontiers in neural circuits · 2025Review
- Molecular Organization of Autonomic, Respiratory, and Spinally-Projecting Neurons in the Mouse Ventrolateral Medulla.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Injury distance limits the transcriptional response to spinal injury.bioRxiv : the preprint server for biology · 2024Article
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- Recent advances in understanding neuronal diversity and neural circuit complexity across different brain regions using single-cell sequencing.Frontiers in neural circuits · 2023Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The mammalian brain contains numerous neurons distributed across forebrain, midbrain, and hindbrain that project axons to the lower spinal cord and work in concert to control movement and achieve homeostasis. Extensive work has mapped the anatomic location of supraspinal cell types and continues to establish specific physiological functions. The patterns of gene expression that typify and distinguish these disparate populations, however, are mostly unknown. Here, using adult mice of mixed sex, we combined retrograde labeling of supraspinal cell nuclei with fluorescence-activated nuclei sorting and single-nuclei RNA sequencing analyses to transcriptionally profile neurons that project axons from the brain to lumbar spinal cord. We identified 14 transcriptionally distinct cell types and used a combination of established and newly identified marker genes to assign an anatomic location to each. To validate the putative marker genes, we visualized selected transcripts and confirmed selective expression within lumbar-projecting neurons in discrete supraspinal regions. Finally, we illustrate the potential utility of these data by examining the expression of transcription factors that distinguish different supraspinal cell types and by surveying the expression of receptors for growth and guidance cues that may be present in the spinal cord. Collectively, these data establish transcriptional differences between anatomically defined supraspinal populations, identify a new set of marker genes of use in future experiments, and provide insight into potential differences in cellular and physiological activity across the supraspinal connectome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.