ArticleMolecular biology of the cell2022
p115RhoGEF activates RhoA to support tight junction maintenance and remodeling.
Article in Molecular biology of the cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Tight junction structure, assembly and (dys)function.Nature reviews. Molecular cell biology · 2026Review
- RhoA/ROCK signaling as a potential convergent pathway in microbiota-gut-brain axis-related cognitive impairment.Frontiers in neurology · 2026Review
- PAK4 promotes vertex remodeling to maintain epithelial integrity and barrier function.bioRxiv : the preprint server for biology · 2025Article
- Neighbor cells restrain furrowing during Xenopus epithelial cytokinesis.Developmental cell · 2025Article
- Anillin tunes contractility and regulates barrier function during Rho flare-mediated tight junction remodeling.Molecular biology of the cell · 2025Article
- Anillin tunes contractility and regulates barrier function during Rho flare-mediated tight junction remodeling.bioRxiv : the preprint server for biology · 2024Article
- Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complex.The Journal of cell biology · 2024Article
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- The COPD GWAS gene ADGRG6 instructs function and injury response in human iPSC-derived type II alveolar epithelial cells.American journal of human genetics · 2023Article
- ZnUMBA - a live imaging method to detect local barrier breaches.Journal of cell science · 2023Article
- Bring the pain: wounding reveals a transition from cortical excitability to epithelial excitability inFrontiers in cell and developmental biology · 2023Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In vertebrates, epithelial cell-cell junctions must rapidly remodel to maintain barrier function as cells undergo dynamic shape-change events. Consequently, localized leaks sometimes arise within the tight junction (TJ) barrier, which are repaired by short-lived activations of RhoA, called "Rho flares." However, how RhoA is activated at leak sites remains unknown. Here we asked which guanine nucleotide exchange factor (GEF) localizes to TJs to initiate Rho activity at Rho flares. We find that p115RhoGEF locally activates Rho flares at sites of TJ loss. Knockdown of p115RhoGEF leads to diminished Rho flare intensity and impaired TJ remodeling. p115RhoGEF knockdown also decreases junctional active RhoA levels, thus compromising the apical actomyosin array and junctional complex. Furthermore, p115RhoGEF is necessary to promote local leak repair to maintain TJ barrier function. In all, our work demonstrates a central role for p115RhoGEF in activating junctional RhoA to preserve barrier function and direct local TJ remodeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.