Evidence map›Paper›PMID 36200416›Full record

ArticleHaematologica2023

CD56bright CD16- natural killer cells as an important regulatory mechanism in chronic graft-

Madeline Lauener, Shima AzadPour, Sayeh Abdossamadi, Vaishnavi Parthasarathy, Bernard Ng, Elena Ostroumov, Geoffrey D E Cuvelier, Megan K Levings, Katherine N MacDonald, Amina Kariminia and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Haematologica, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Madeline LauenerMichael Cuccione Childhood Cancer Research Program, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver.
Shima AzadPourDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran.
Sayeh AbdossamadiMichael Cuccione Childhood Cancer Research Program, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver.
Vaishnavi ParthasarathyMichael Cuccione Childhood Cancer Research Program, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver.
Bernard NgMichael Cuccione Childhood Cancer Research Program, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver.
Elena OstroumovMichael Cuccione Childhood Cancer Research Program, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver.
Geoffrey D E CuvelierCancerCare Manitoba, University of Manitoba, Winnipeg, MB.
Megan K LevingsBritish Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, BC; School of Biomedical Engineering, University of British Columbia, Vancouver, BC; Department of Surgery, University of British Columbia, Vancouver.
Katherine N MacDonaldBritish Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, BC; School of Biomedical Engineering, University of British Columbia, Vancouver, BC; Michael Smith Laboratories, University of British Columbia, Vancouver.
Amina KariminiaMichael Cuccione Childhood Cancer Research Program, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver.
Kirk R SchultzMichael Cuccione Childhood Cancer Research Program, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver. kschultz@mail.ubc.ca.
BC Children's Hospital · CAUniversity of British Columbia · CACanada's Michael Smith Genome Sciences Centre · CATarbiat Modares University · IRUniversity of Manitoba · CA

Funding

CIHR
6 · The paper itself

Abstract

Chronic graft-versus-host disease (cGvHD) is a major cause of morbidity after hematopoietic stem cell transplantation (HSCT). In large patient populations, we have shown a CD56bright natural killer (NK) population to strongly associate with a lack of cGvHD and we hypothesize that these cells function to suppress cGvHD. We aimed to isolate and define the characteristics of regulatory NK (NKreg) cells associated with suppression of cGvHD. Immunophenotypic evaluation of a large pediatric population found the CD56bright NK population associated with a lack of cGvHD to be perforin-, Granzyme B-, and CD335+. Transcriptome analysis of a small patient cohort of CD56bright compared to CD56dim NK cells found the NKreg cells to also overexpress Granzyme K, IL-7R, GPR183, RANK, GM-CSFR, TCF7, and IL23A. Further analysis of this CD56bright NKreg population found a subpopulation that overexpressed IRF1, and TNF. We also found that viable NKreg cells may be isolated by sorting on CD56+ and CD16- NK cells, and this population can suppress allogeneic CD4+ T cells, but not Treg cells or CD8+ T cells through a non-cytolytic, cell-cell contact dependent mechanism. Suppression was not reliant upon the NKp44, NKp46, or GPR183 receptors. Additionally, NKreg cells do not kill leukemic cells. Moreover, this is the first paper to clearly establish that a CD56brightCD3-CD16-perforin- NKreg population associates with a lack of cGvHD and has several unique characteristics, including the suppression of helper T-cell function in vitro. With further investigation we may decipher the mechanism of NKreg suppression and operationalize expansion of NKreg cells associated with cGvHD suppression.

Indexed as

Bronchiolitis Obliterans SyndromeGraft vs Host DiseaseCD56 AntigenChildChronic DiseaseHumansKiller Cells, NaturalPerforinT-Lymphocytes, RegulatoryCD56 AntigenPerforin

Identifiers

PMID36200416
PMCPMC9973474
OpenAlexW4302287941

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.