ArticleScientific reports2022
Contribution of autofluorescence from intracellular proteins in multiphoton fluorescence lifetime imaging.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- FLIM Characterization of Staurosporine-Induced Changes in Endogenous Cellular Autofluorescence.Chemical & biomedical imaging · 2026Article
- Carbon Dots and Mitochondria-Advances in Targeting, Imaging, and Therapeutics.International journal of molecular sciences · 2026Review
- Challenges and Prospects of Using Novel Nonlinear Effects in Multimode Optical Fibers for Multiphoton Endomicroscopy.Diagnostics (Basel, Switzerland) · 2026Review
- Modeling spectroradiometric measurements of oral mucosal tissue autofluorescence.Biomedical optics express · 2026Article
- Biomedical optical imaging of acne pathology: from single-modality methods to multimodal integration.Frontiers in medicine · 2026Review
- Label-free human skin imaging with enhanced molecular contrast via time-resolved fluorescence and advanced phasor analysis.Communications biology · 2025Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.eLife · 2025Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.bioRxiv : the preprint server for biology · 2025Article
- Multimodal Optical Imaging Platform for Studying Cellular Metabolism.Journal of visualized experiments : JoVE · 2025Article
- Exploring MPM-FLIM for diagnostics of porokeratosis - a pilot studyBiomedical optics express · 2025Article
- Insights into metabolic changes during epidermal differentiation as revealed by multiphoton microscopy with fluorescence lifetime imaging.Scientific reports · 2025Article
- Investigating the relationship between hypoxia, hypoxia-inducible factor 1, and the optical redox ratio in response to radiation therapy.Biophotonics discovery · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
- Quantitative multiphoton imaging of cell metabolism, stromal fibers, and keratinization enables label-free discrimination of esophageal squamous cell carcinoma.Biomedical optics express · 2023Article
- Imaging guidance for cholesteatoma surgery using tissue autofluorescence.Journal of biomedical optics · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) is extensively proposed as a non-invasive optical method to study tissue metabolism. The approach is based on recording changes in the fluorescence lifetime attributed to metabolic co-enzymes, of which nicotinamide adenine dinucleotide (NADH) is of major importance. However, intrinsic tissue fluorescence is complex. Particularly when utilizing two-photon excitation, as conventionally employed in MPM. This increases the possibility for spectral crosstalk and incorrect assignment of the origin of the FLIM signal. Here we demonstrate that in keratinocytes, proteins such as keratin may interfere with the signal usually assigned to NADH in MPM-FLIM by contributing to the lifetime component at 1.5 ns. This is supported by a change in fluorescence lifetime distribution in KRT5- and KRT14-silenced cells. Altogether, our results suggest that the MPM-FLIM data originating from cellular autofluorescence is far more complex than previously suggested and that the contribution from other tissue constituents should not be neglected-changing the paradigm for data interpretation in this context.
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