Evidence map›Paper›PMID 36197102›Full record

ArticleApplied and environmental microbiology2022

Balancing Trade-Offs Imposed by Growth Media and Mass Spectrometry for Bacterial Exometabolomics.

Ann E Donnelly, Nithya Narayanan, Caroline M C Birer-Williams, Travis J DeWolfe, Rosalie K Chu, Christopher R Anderton, Erik S Wright

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ann E DonnellyDepartment of Biomedical Informatics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.ORCID 0000-0002-6296-5401
Nithya NarayananDepartment of Biomedical Informatics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.
Caroline M C Birer-WilliamsDepartment of Biomedical Informatics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.ORCID 0000-0001-7204-2013
Travis J DeWolfeDepartment of Biomedical Informatics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.ORCID 0000-0001-6261-1235
Rosalie K ChuEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratorygrid.451303.0, Richland, Washington, USA.
Christopher R AndertonEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratorygrid.451303.0, Richland, Washington, USA.ORCID 0000-0002-6170-1033
Erik S WrightDepartment of Biomedical Informatics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.ORCID 0000-0002-1457-4019

Funding

The internship in Biomedical Research, Informatics, and Computer Science (iBRIC): Biomedical Informatics and Data Science research experiences for students from Minority Serving InstitutionsT15LM007059 · NLM · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HARRY S HOCHHEISER · 1987 to 2026
$23.9M
Uncovering synergistic antibiotic cocktails with comparative genomicsDP2AI145058 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WRIGHT, ERIK SCOTT · 2018 to 2018
$2.3M
NIAID NIH HHS DP2 AI145058NLM NIH HHS T15 LM007059
6 · The paper itself

Abstract

The bacterial exometabolome consists of a vast array of specialized metabolites, many of which are only produced in response to specific environmental stimuli. For this reason, it is desirable to control the extracellular environment with a defined growth medium composed of pure ingredients. However, complex (undefined) media are expected to support the robust growth of a greater variety of microorganisms than defined media. Here, we investigate the trade-offs inherent to a range of complex and defined solid media for the growth of soil microorganisms, production of specialized metabolites, and detection of these compounds using direct infusion mass spectrometry. We find that complex media support growth of more soil microorganisms, as well as allowing for the detection of more previously discovered natural products as a fraction of total

Indexed as

Biological ProductsStreptomycesMass SpectrometrySoilBiological ProductsSoilcultivation conditionsDIMSexometabolomeLESA-MSnatural productsspecialized metabolitesStreptomyces

Identifiers

PMID36197102
PMCPMC9599359

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.