ArticleBMC genomics2022
High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes.
Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Deciphering the complex molecular architecture of the genetically modified soybean FG72 through paired-end whole genome sequencing.Food chemistry. Molecular sciences · 2025Article
- Camelina circRNA landscape: Implications for gene regulation and fatty acid metabolism.The plant genome · 2025Article
- Article
- Review of the technology used for structural characterization of the GMO genome using NGS data.Genomics & informatics · 2024Article
- Characterization of regulatory genesFrontiers in microbiology · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundGenetic engineering of crop plants has been successful in transferring traits into elite lines beyond what can be achieved with breeding techniques. Introduction of transgenes originating from other species has conferred resistance to biotic and abiotic stresses, increased efficiency, and modified developmental programs. The next challenge is now to combine multiple transgenes into elite varieties via gene stacking to combine traits. Generating stable homozygous lines with multiple transgenes requires selection of segregating generations which is time consuming and labor intensive, especially if the crop is polyploid. Insertion site effects and transgene copy number are important metrics for commercialization and trait efficiency.
resultsWe have developed a simple method to identify the sites of transgene insertions using T-DNA-specific primers and high-throughput sequencing that enables identification of multiple insertion sites in the T
conclusionThis new methodology enables the early selection of desirable transgene location and copy number to generate homozygous lines within two generations.
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Registered trials
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