ArticleCurrent protocols2022
Maize Seedling Growth and Hormone Response Assays Using the Rolled Towel Method.
Article in Current protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- High-Throughput Root Phenotyping of Maize Seedlings.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Harnessing Multifunctional Bacillus thuringiensis for Plant Growth Promotion and Biocontrol Potential Against Spodoptera frugiperda.Journal of basic microbiology · 2026Article
- GrowScreen-Rhizo 3 - automated large-scale high throughput greenhouse phenotyping of plant root and shoot development.Plant phenomics (Washington, D.C.) · 2026Article
- Article
- KATANIN promotes cell elongation and division to generate proper cell numbers in maize organs.Nature communications · 2026Article
- Transcriptomics Reveals Cold Tolerance Maize Lines Involved in the Phenylpropanoid and Flavonoid Pathways.Plants (Basel, Switzerland) · 2026Article
- Identification of phenotypic and transcriptomic signatures underpinning maize crown root systems.Plant phenomics (Washington, D.C.) · 2025Article
- ZmPILS6 is an auxin efflux carrier required for maize root morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Roles of auxin pathways in maize biology.Journal of experimental botany · 2023Review
- A Plate Growth Assay to Quantify Embryonic Root Development ofBio-protocol · 2023Article
- Maize Seedling Growth and Hormone Response Assays Using the Rolled Towel Method.Current protocols · 2022Article
- The Invisible Frontline: High-Tech Root Imaging for Crop Stress Adaptation.Physiologia plantarumReview
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Root system architecture is a critical factor in maize health and stress resilience. Determining the genetic and environmental factors that shape maize root system architecture is an active research area. However, the ability to phenotype juvenile root systems is hindered by the use of field-grown and soil-based systems. An alternative to soil- and field-based growing conditions for maize seedlings is a controlled environment with a soil-free medium, which can facilitate root system phenotyping. Here, we describe how to grow maize under soil-free conditions for up to 12 days to facilitate root phenotyping. Maize seeds are sterilized and planted on specialized seed germination paper to minimize fungal contamination and ensure synchronized seedling growth, followed by imaging at the desired time point. The root images are then analyzed to quantify traits of interest, such as primary root length, lateral root density, seminal root length, and seminal root number. In addition, juvenile shoot traits can be quantified using manual annotation methods. We also outline the steps for performing rigorous hormone response assays for four classical phytohormones: auxin, brassinosteroid, cytokinin, and jasmonic acid. This protocol can be rapidly scaled up and is compatible with genetic screens and sample collection for downstream molecular analyses such as transcriptomics and proteomics. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Maize seedling rolled towel assay and phenotyping Basic Protocol 2: Maize seedling hormone response assays using the rolled towel assay.
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