ArticleBiotechnology for biofuels and bioproducts2022
Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock.
Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 64 citations in OpenAlex.
- Utilization, metabolic regulation and applications of hydrophobic substrates inSynthetic and systems biotechnology · 2026Review
- Yeast species resource exploration and application in cell factories.Synthetic and systems biotechnology · 2026Review
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- 10 years of CRISPR/CAS genomic engineering in Yarrowia lipolytica.Bioprocess and biosystems engineering · 2026Review
- Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction inSynthetic and systems biotechnology · 2026Article
- Construction and analysis of a cell factory for terpenoid biosynthesis inSynthetic and systems biotechnology · 2026Article
- EngineeringSynthetic and systems biotechnology · 2026Article
- Metabolic Engineering of Yeasts: A Key Cell Factory Platform for Advanced Biomanufacturing.Journal of fungi (Basel, Switzerland) · 2025Article
- EngineeringSynthetic and systems biotechnology · 2025Article
- Expanding the Terpene Universe: Synthetic Biology and Non-Natural Chemistry in Engineered Microorganisms.Molecules (Basel, Switzerland) · 2025Review
- Elucidating the mechanisms of echinocandin B biosynthesis under fatty acid feeding in3 Biotech · 2025Article
- Combinatorial metabolic engineering of Yarrowia lipolytica for high-level production of the plant-derived diterpenoid sclareol.Microbial cell factories · 2025Article
- Engineering Yarrowia lipolytica to Enhance the Production of Malonic Acid via Malonyl-CoA Pathway at High Titer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- Developing polycistronic expression tool inSynthetic and systems biotechnology · 2025Article
- Fine-Tuning the Function of Farnesene Synthases for Selective Synthesis of Farnesene Stereoisomers.Journal of agricultural and food chemistry · 2024Article
- Metabolic engineering of Saccharomyces cerevisiae for high-level production of (+)-ambrein from glucose.Biotechnology letters · 2024Article
- Biosynthesis Progress of High-Energy-Density Liquid Fuels Derived from Terpenes.Microorganisms · 2024Review
- Genome-scale transcriptional activation by non-homologous end joining-mediated integration in Yarrowia lipolytica.Biotechnology for biofuels and bioproducts · 2024Article
- Systematic metabolic engineering ofFrontiers in bioengineering and biotechnology · 2024Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundβ-Farnesene is a sesquiterpene with versatile industrial applications. The production of β-farnesene from waste lipid feedstock is an attractive method for sustainable production and recycling waste oil. Yarrowia lipolytica is an unconventional oleaginous yeast, which can use lipid feedstock and has great potential to synthesize acetyl-CoA-derived chemicals.
resultsIn this study, we engineered Y. lipolytica to produce β-farnesene from lipid feedstock. To direct the flux of acetyl-CoA, which is generated from lipid β-oxidation, to β-farnesene synthesis, the mevalonate synthesis pathway was compartmentalized into peroxisomes. β-Farnesene production was then engineered by the protein engineering of β-farnesene synthase and pathway engineering. The regulation of lipid metabolism by enhancing β-oxidation and eliminating intracellular lipid synthesis was further performed to improve the β-farnesene synthesis. As a result, the final β-farnesene production with bio-engineering reached 35.2 g/L and 31.9 g/L using oleic acid and waste cooking oil, respectively, which are the highest β-farnesene titers reported in Y. lipolytica.
conclusionsThis study demonstrates that engineered Y. lipolytica could realize the sustainable production of value-added acetyl-CoA-derived chemicals from waste lipid feedstock.
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