ArticleNature communications2022
Systematic dissection of key factors governing recombination outcomes by GCE-SCRaMbLE.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- rDNA-mediated multicopy integration and gene dosage quantification system for microbial zeaxanthin biosynthesis.World journal of microbiology & biotechnology · 2026Article
- EASY-C: Extraction and Analysis of Small Yeast Chromosomes-A rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids fromSynthetic biology (Oxford, England) · 2026Article
- Systematic Engineering for Efficient Uric Acid-Degrading Activity in Probiotic YeastACS synthetic biology · 2025Article
- Genetic Code Expansion: Recent Developments and Emerging Applications.Chemical reviews · 2025Review
- Golden Gate Cloning for Efficient Biosynthesis of Lycopene in Synthetic Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- CRISPR-Mediated rDNA Integration and Fluorescence Screening for Pathway Optimization inChem & bio engineering · 2024Article
- Recent advances in the expanding genetic code.Current opinion in chemical biology · 2024Review
- The de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging.Nature communications · 2024Article
- Enhancing whole yeast genome rearrangements through multiple LoxPsym sequences.Science China. Life sciences · 2024Article
- Synthetic Genomics: Repurposing Biological Systems for Applications in Engineering Biology.ACS synthetic biology · 2024Review
- Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts.Nature communications · 2024Article
- Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae.Nature communications · 2024Article
- Article
- Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast.Cell genomics · 2023Article
- A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid.Nature communications · 2023Article
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- Dynamics of synthetic yeast chromosome evolution shaped by hierarchical chromatin organization.National science review · 2023Article
- Investigation of Genome Biology by Synthetic Genome Engineering.Bioengineering (Basel, Switzerland) · 2023Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the completion of Sc2.0 chromosomes, synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) becomes more critical for in-depth investigation of fundamental biological questions and screening of industrially valuable characteristics. Further applications, however, are hindered due to the lack of facile and tight regulation of the SCRaMbLE process, and limited understanding of key factors that may affect the rearrangement outcomes. Here we propose an approach to precisely regulate SCRaMbLE recombination in a dose-dependent manner using genetic code expansion (GCE) technology with low basal activity. By systematically analyzing 1380 derived strains and six yeast pools subjected to GCE-SCRaMbLE, we find that Cre enzyme abundance, genome ploidy and chromosome conformation play key roles in recombination frequencies and determine the SCRaMbLE outcomes. With these insights, the GCE-SCRaMbLE system will serve as a powerful tool in the future exploitation and optimization of the Sc2.0-related technologies.
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