ArticleProceedings of the National Academy of Sciences of the United States of America2022
Mesoscale structure-function relationships in mitochondrial transcriptional condensates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- Condensates and cell states: A new paradigm for understanding tumor biology.Biophysical journal · 2026Review
- Biomolecular condensates can function as inherent catalysts.Molecular cell · 2026Article
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Membranes arrest the coarsening of mitochondrial condensates in human cells.Communications biology · 2026Article
- Fluorescence-based mapping of condensate dielectric permittivity uncovers hydrophobicity-driven membrane interactions.Nature communications · 2026Article
- Learning nature's assembly language with polymers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Transient pH changes drive vacuole formation in enzyme-polymer condensates.Nature chemical engineering · 2026Article
- Deciphering the molecular mechanisms of FET fusion oncoprotein-DNA hollow co-condensates.Nature communications · 2025Article
- A Perspective on the Role of Mitochondrial Biomolecular Condensates (mtBCs) in Neurodegenerative Diseases and Evolutionary Links to Bacterial BCs.International journal of molecular sciences · 2025Review
- Phase separation in mitochondrial fate and mitochondrial diseases.Proceedings of the National Academy of Sciences of the United States of America · 2025Review
- Condenzymes: Biomolecular condensates with inherent catalytic activities.bioRxiv : the preprint server for biology · 2025Article
- Spatial analysis of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress.Science advances · 2025Article
- Differential interactions determine anisotropies at interfaces of RNA-based biomolecular condensates.Nature communications · 2025Article
- Backbone-mediated weakening of pairwise interactions enables percolation in peptide-based mimics of protein condensates.Communications chemistry · 2025Article
- Single-fluorogen imaging reveals distinct environmental and structural features of biomolecular condensates.Nature physics · 2025Article
- Emergent microenvironments of nucleoli.Nucleus (Austin, Tex.) · 2024Review
- Article
- A spatial atlas of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress.bioRxiv : the preprint server for biology · 2024Article
- Fundamental Aspects of Phase-Separated Biomolecular Condensates.Chemical reviews · 2024Review
- Biomolecular condensates form spatially inhomogeneous network fluids.Nature communications · 2024Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In live cells, phase separation is thought to organize macromolecules into membraneless structures known as biomolecular condensates. Here, we reconstituted transcription in condensates from purified mitochondrial components using optimized in vitro reaction conditions to probe the structure-function relationships of biomolecular condensates. We find that the core components of the mt-transcription machinery form multiphasic, viscoelastic condensates in vitro. Strikingly, the rates of condensate-mediated transcription are substantially lower than in solution. The condensate-mediated decrease in transcriptional rates is associated with the formation of vesicle-like structures that are driven by the production and accumulation of RNA during transcription. The generation of RNA alters the global phase behavior and organization of transcription components within condensates. Coarse-grained simulations of mesoscale structures at equilibrium show that the components stably assemble into multiphasic condensates and that the vesicles formed in vitro are the result of dynamical arrest. Overall, our findings illustrate the complex phase behavior of transcribing, multicomponent condensates, and they highlight the intimate, bidirectional interplay of structure and function in transcriptional condensates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.