ArticlePLoS biology2022
Heliorhodopsin binds and regulates glutamine synthetase activity.
Article in PLoS biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Ecological success in freshwater lakes: insights from novel cultivated lineages of the abundant Nanopelagicales order.Microbiome · 2025Article
- Salinity-driven niche differentiation within the aquatic Luna-1 subcluster.ISME communications · 2025Article
- Molecular properties of a viral heliorhodopsin, V2HeR2.Biophysics and physicobiology · 2025Article
- Solid-state NMR of the retinal protonated Schiff base in microbial rhodopsins.Magnetic resonance letters · 2024Review
- Proton-pumping photoreceptor controls expression of ABC transporter by regulating transcription factor through light.Communications biology · 2024Article
- Molecular Mechanisms behind Circular Dichroism Spectral Variations between Channelrhodopsin and Heliorhodopsin Dimers.The journal of physical chemistry letters · 2024Article
- Heliorhodopsin-mediated light-modulation of ABC transporter.Nature communications · 2024Article
- pH-Dependent Binding and Releasing Mechanism of Acetate in the Inner Water Cavity of Heliorhodopsin.Biochemistry · 2023Article
- Flotillin-associated rhodopsin (FArhodopsin), a widespread paralog of proteorhodopsin in aquatic bacteria with streamlined genomes.mSystems · 2023Article
- Recent advances in signaling and activation mechanism in microbial rhodopsins: Report for the session 6 at the 19Biophysics and physicobiology · 2023Article
- Proton transfer and conformational changes along the hydrogen bond network in heliorhodopsin.Communications biology · 2022Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photoreceptors are light-sensitive proteins found in various organisms that respond to light and relay signals into the cells. Heliorhodopsin, a retinal-binding membrane protein, has been recently discovered, however its function remains unknown. Herein, we investigated the relationship between Actinobacteria bacterium IMCC26103 heliorhodopsin (AbHeR) and an adjacent glutamine synthetase (AbGS) in the same operon. We demonstrate that AbHeR binds to AbGS and regulates AbGS activity. More specifically, the dissociation constant (Kd) value of the binding between AbHeR and AbGS is 6.06 μM. Moreover, the absence of positively charged residues within the intracellular loop of AbHeR impacted Kd value as they serve as critical binding sites for AbGS. We also confirm that AbHeR up-regulates the biosynthetic enzyme activity of AbGS both in vitro and in vivo in the presence of light. GS is a key enzyme involved in nitrogen assimilation that catalyzes the conversion of glutamate and ammonia to glutamine. Hence, the interaction between AbHeR and AbGS may be critical for nitrogen assimilation in Actinobacteria bacterium IMCC26103 as it survives in low-nutrient environments. Overall, the findings of our study describe, for the first time, to the best of our knowledge, a novel function of heliorhodopsin as a regulatory rhodopsin with the capacity to bind and regulate enzyme activity required for nitrogen assimilation.
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