ArticleFrontiers in cellular and infection microbiology2022
The diagnostic value of next-generation sequencing technology in sepsis.
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Effects of a clinical metagenomics intervention on clinical outcomes, healthcare costs, and health-related quality of life in patients with sepsis or septic shock: results of the randomized-controlled DigiSep trial.Intensive care medicine · 2026Trial
- The diagnostic role of albumin-based ratio markers for periprosthetic joint infection.Frontiers in cellular and infection microbiology · 2026Article
- Article
- Leveraging laboratory biomarkers to predict urosepsis after upper urinary tract stone surgery: an explainable machine learning approach.BMC medical informatics and decision making · 2025Article
- Neonatal and pediatric sepsis: Microbiological insights, diagnostic innovations, and antimicrobial challenges.World journal of clinical pediatrics · 2025Review
- Comparison of metagenomic next-generation sequencing (mNGS) technology with routine laboratory culture for bacterial and fungal detection in bronchoalveolar lavage fluid.Journal of thoracic disease · 2025Article
- Metagenomic next-generation sequencing (mNGS) guides targeted antibiotic therapy and reduces mortality in sepsis: a propensity-matched retrospective cohort study.BMC infectious diseases · 2025Article
- Reassessing the promise of NGS in sepsis diagnosis: overcoming scalability barriers.Future microbiology · 2025Article
- Development of a Multiplexed qPCR Kit for the Detection of Bloodstream Infection.Journal of clinical laboratory analysis · 2025Article
- Plasma microbial cell-free DNA characterization in different populations based on the droplet digital PCR method: a multi-cohort study.Frontiers in microbiology · 2025Article
- Next-generation sequencing guides the treatment of severe community-acquired pneumonia with empiric antimicrobial therapy failure: A propensity-score-matched study.PLoS neglected tropical diseases · 2024Article
- Use of pus metagenomic next-generation sequencing for efficient identification of pathogens in patients with sepsis.Folia microbiologica · 2024Article
- Utility of pan-bacterial and pan-fungal PCR in endophthalmitis: case report and review of the literature.Journal of ophthalmic inflammation and infection · 2024Review
- The Value of Next-Generation Sequencing in Diagnosis and Therapy of Critically Ill Patients with Suspected Bloodstream Infections: A Retrospective Cohort Study.Journal of clinical medicine · 2024Article
- Incorporating anti-infective drugs into peripherally inserted catheters does not reduce infection rates in neonates.Frontiers in pediatrics · 2023Article
- Performance of next-generation sequencing for diagnosis of blood infections byFrontiers in cellular and infection microbiology · 2023Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Objective: This study aims to assess the clinical utility of next-generation sequencing (NGS) in sepsis diagnosis. Methods: A prospective study was conducted on patients with a high suspicion of sepsis by unknown pathogens from January 2017 to December 2021. Blood samples were taken from patients to perform NGS, blood culture (BC), leucocyte (WBC), procalcitonin (PCT), creatinine (CREA), Albumin (ALB) and C-reactive protein (CRP) tests. Results: The feedback time for BC was 3~5 days for bacteria and 5~7 days for fungi, while the turnover time for NGS was only 24 h. The clinical diagnosis was considered the "gold standard". 83 patients passed our inclusion criteria and were separated into two groups by clinical diagnosis. 62 met the clinical diagnosis criteria for sepsis and 21 were non-sepsis. The data from the two groups were retrospectively compared and analyzed. Of 62 sepsis in 83 patients, 8(9.64%) were diagnosed by both BC and NGS, 51 (61.45%) by NGS only, 1(1.20%) by BC and 2 (2.41%) by conventional testing only; PCT, CREA, CRP levels and the detection rate of NGS and BC were higher in the sepsis group than in the non-sepsis group, while ALB levels were lower (p<0.05). The logistic regression results in our study revealed that NGS and ALB were independent prediction factors for sepsis ( Conclusion: NGS can effectively and quickly identify pathogens, thereby emerges as a promising technology for sepsis diagnosis. Combination of NGS and ALB can be used for early screening and is more powerful than NGS or ALB only.
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