Evidence map›Paper›PMID 36188118›Full record

ArticleBiotechnology reports (Amsterdam, Netherlands)2022

Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production.

Neelam Mahala, Aastha Mittal, Manohar Lal, Uma S Dubey

Abstract read
In one paragraph

Article in Biotechnology reports (Amsterdam, Netherlands), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Neelam MahalaDepartment of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, India.
Aastha MittalDepartment of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, India.
Manohar LalDepartment of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, India.
Uma S DubeyDepartment of Biological Sciences, Birla Institute of Technology of Science, Pilani (BITS Pilani), Pilani Campus, Rajasthan 333031, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present article exemplifies a novel method to isolate highly purified bioactive lactoferrin from camel milk. Cytotoxicity of lactoferrin against the Hela cells was used to evaluate its bioactivity. SDS-PAGE and LC-MS analysis was done for its identification and characterization. The purified camel milk lactoferrin was found to be 708 amino acids in length with a molecular weight of 77.3 kDa and a pI value of 8.24. This pH-dependent isolation procedure ensures the retention of bioactive lactoferrin from camel milk. The importance of the present work lies in its simplicity and scalability for manufacturing bioactive lactoferrin at an industrial level.

Indexed as

Camel milkCytotoxicity assayLactoferrinLiquid chromatography-mass spectrometry (LC-MS)OD, Optical densitypH-dependent isolationrpm, Revolutions per minuteWheyαs2-CN, αS2-Casein

Identifiers

PMID36188118
PMCPMC9519473

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.