ArticleBiomedical optics express2022
Axial scanning of dual focus to improve light sheet microscopy.
Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Line-scan chromatic confocal microscopy for multi-depth imaging of skin tissue using deconvolution.Biomedical optics express · 2025Article
- Remote refocusing for multi-scale imaging.Journal of biomedical optics · 2024Article
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Nature communications · 2024Article
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Research square · 2023Article
- Sidelobe suppression in structured light sheet fluorescence microscopy by the superposition of two light sheets.Biomedical optics express · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Axially swept light sheet microscopy (ASLM) is an emerging technique that enables isotropic, subcellular resolution imaging with high optical sectioning capability over a large field-of-view (FOV). Due to its versatility across a broad range of immersion media, it has been utilized to image specimens that may range from live cells to intact chemically cleared organs. However, because of its design, the performance of ASLM-based microscopes is impeded by a low detection signal and the maximum achievable frame-rate for full FOV imaging. Here we present a new optical concept that pushes the limits of ASLM further by scanning two staggered light sheets and simultaneously synchronizing the rolling shutter of a scientific camera. For a particular peak-illumination-intensity, this idea can make ASLMs image twice as fast without compromising the detection signal. Alternately, for a particular frame rate our method doubles the detection signal without requiring to double the peak-illumination-power, thereby offering a gentler illumination scheme compared to tradition single-focus ASLM. We demonstrate the performance of our instrument by imaging fluorescent beads and a PEGASOS cleared-tissue mouse brain.
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Registered trials
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