ArticleFrontiers in bioengineering and biotechnology2022
A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems.
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Microbial cell-free protein synthesis and its progression toward industrial use.Microbiology (Reading, England) · 2026Review
- Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity.NPJ microgravity · 2024Article
- A Cost-EffectiveBioengineering (Basel, Switzerland) · 2024Article
- Review
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9 authors.
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Abstract
Cell-free gene expression (CFE) systems are an attractive tool for engineering within synthetic biology and for industrial production of high-value recombinant proteins. CFE reactions require a cell extract, energy system, amino acids, and DNA, to catalyse mRNA transcription and protein synthesis. To provide an amino acid source, CFE systems typically use a commercial standard, which is often proprietary. Herein we show that a range of common microbiology rich media (i.e., tryptone, peptone, yeast extract and casamino acids) unexpectedly provide an effective and low-cost amino acid source. We show that this approach is generalisable, by comparing batch variability and protein production in the following range of CFE systems:
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