Evidence map›Paper›PMID 36184586›Full record

ArticleParasites & vectors2022

Excretory-secretory products from the brown stomach worm, Teladorsagia circumcincta, exert antimicrobial activity in in vitro growth assays.

James Rooney, Timothy L Williams, Holly M Northcote, Fiona E Karet Frankl, Daniel R G Price, Alasdair J Nisbet, Russell M Morphew, Cinzia Cantacessi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

James RooneyDepartment of Veterinary Medicine, University of Cambridge, Cambridge, CB3 0ES, UK.
Timothy L WilliamsDepartment of Veterinary Medicine, University of Cambridge, Cambridge, CB3 0ES, UK.
Holly M NorthcoteInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, SY23 2DA, UK.
Fiona E Karet FranklDepartment of Medical Genetics, University of Cambridge, Cambridge, CB2 0QQ, UK.
Daniel R G PriceVaccines and Diagnostics Department, Moredun Research Institute, Penicuik, EH26 0PZ, UK.
Alasdair J NisbetVaccines and Diagnostics Department, Moredun Research Institute, Penicuik, EH26 0PZ, UK.
Russell M MorphewInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, SY23 2DA, UK.
Cinzia CantacessiDepartment of Veterinary Medicine, University of Cambridge, Cambridge, CB3 0ES, UK. cc779@cam.ac.uk.
University of Cambridge · GBMoredun Research Institute · GBAberystwyth University · GBInstitute of Biological, Environmental and Rural Sciences · GB

Funding

Biotechnology and Biological Sciences Research Council BB/L027186/1
6 · The paper itself

Abstract

backgroundOver the past decade, evidence has emerged of the ability of gastrointestinal (GI) helminth parasites to alter the composition of the host gut microbiome; however, the mechanism(s) underpinning such interactions remain unclear. In the current study, we (i) undertake proteomic analyses of the excretory-secretory products (ESPs), including secreted extracellular vesicles (EVs), of the 'brown stomach worm' Teladorsagia circumcincta, one of the major agents causing parasite gastroenteritis in temperate areas worldwide; (ii) conduct bioinformatic analyses to identify and characterise antimicrobial peptides (AMPs) with putative antimicrobial activity; and (iii) assess the bactericidal and/or bacteriostatic properties of T. circumcincta EVs, and whole and EV-depleted ESPs, using bacterial growth inhibition assays.

methodsSize-exclusion chromatography was applied to the isolation of EVs from whole T. circumcincta ESPs, followed by EV characterisation via nanoparticle tracking analysis and transmission electron microscopy. Proteomic analysis of EVs and EV-depleted ESPs was conducted using liquid chromatography-tandem mass spectrometry, and prediction of putative AMPs was performed using available online tools. The antimicrobial activities of T. circumcincta EVs and of whole and EV-depleted ESPs against Escherichia coli were evaluated using bacterial growth inhibition assays.

resultsSeveral molecules with putative antimicrobial activity were identified in both EVs and EV-depleted ESPs from adult T. circumcincta. Whilst exposure of E. coli to whole ESPs resulted in a significant reduction of colony-forming units over 3 h, bacterial growth was not reduced following exposure to worm EVs or EV-depleted ESPs.

conclusionsOur data points towards a bactericidal and/or bacteriostatic function of T. circumcincta ESPs, likely mediated by molecules with antimicrobial activity.

Indexed as

Anti-Infective AgentsIntestinal Diseases, ParasiticAnimalsEscherichia coliOstertagiaProteomicsStomachAnti-Infective AgentsAntimicrobial peptideExcretory-secretory productsExtracellular vesicleGastrointestinal helminthMicrobiomeRuminantTeladorsagia circumcincta

Identifiers

PMID36184586
PMCPMC9528173
OpenAlexW4300003427

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.