ArticleAnalytical and bioanalytical chemistry2022
A sensitive LC-MS/MS method for isomer separation and quantitative determination of 51 pyrrolizidine alkaloids and two tropane alkaloids in cow's milk.
Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Characterization of three new antiproliferative shikimic acid derivatives and known pyrrolizidine alkaloids from Senecio oleosus guided by diagnostic ions and molecular networking.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- DDA/DIA mass spectrometry coupled with molecular networking and machine learning: high-coverage profiling of pyrrolizidine alkaloids.Analytical and bioanalytical chemistry · 2026Article
- Limited Impact of Column Chemistry and Length on Proteome Coverage Under High-Speed DIA.Molecular & cellular proteomics : MCP · 2026Article
- A validated LC-MS/MS multi-method for the determination of 110 mycotoxins and plant toxins in cow milk and application to samples from Germany.Analytical and bioanalytical chemistry · 2025Article
- Quantitative Analysis of Pyrrolizidine Alkaloids in Food Matrices and Plant-Derived Samples Using UHPLC-MS/MS.Foods (Basel, Switzerland) · 2025Article
- Alkaloid profile of Italian alpine milk.Journal of food science · 2025Article
- Article
- Pyrrolizidine Alkaloids as Hazardous Toxins in Natural Products: Current Analytical Methods and Latest Legal Regulations.Molecules (Basel, Switzerland) · 2024Review
- Analysis of Pyrrolizidine Alkaloids in Stingless Bee Honey and Identification of a Botanical Source asToxins · 2024Article
- Assessment of Toxic Pyrrolizidine and Tropane Alkaloids in Herbal Teas and Culinary Herbs Using LC-Q-ToF/MS.Foods (Basel, Switzerland) · 2023Article
- Pyrrolizidine Alkaloid Extraction and Analysis: Recent Updates.Foods (Basel, Switzerland) · 2022Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
Abstract
1,2-Unsaturated pyrrolizidine alkaloids (PA), their corresponding N-oxides (PANO), and tropane alkaloids (TA) are toxic secondary plant metabolites. Their possible transfer into the milk of dairy cows has been studied in feeding trials; however, only few data on the occurrence of these toxins in milk are available. In this study, the development of a sensitive analytical approach for the simultaneous detection and quantification of a broad range of 54 PA/PANO as well as of the TA atropine and scopolamine in milk of dairy cows is presented. The method optimisation focused on sensitivity and separation of PA/PANO isomers. Milk samples were extracted using liquid-liquid extraction with aqueous formic acid and n-hexane, followed by a cation-exchange solid-phase extraction for purification. Reversed phase liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis was performed using alkaline solvent conditions. Validation proved low limits of detection and quantification of 0.005 to 0.054 µg/L and of 0.009 to 0.123 µg/L, respectively. For 51 of the 54 tested PA/PANO and both TA, the recovery rates ranged from 64 to 127% with repeatability (RSD
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