ArticleAnnals of biomedical engineering2023
Cryopreservation of Whole Rat Livers by Vitrification and Nanowarming.
Article in Annals of biomedical engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- Magnetic induction heating: where will the field go next?Chemical Society reviews · 2026Review
- Mathematical Modeling of Liver Metabolic Activity Under Ex Vivo Conditions upon Exposure to Magnetic Nanoparticles.Biomedicines · 2026Article
- Low-toxicity vitrification of small-diameter arterial grafts by achieving a Cryo-Stasis state.Materials today. Bio · 2026Article
- The evolution of liver graft preservation strategies in liver transplantation.Hepatobiliary surgery and nutrition · 2026Article
- In vivo toxicity assessment of silica-coated iron oxide nanoparticles for nanowarming in organ cryopreservation.Journal of nanobiotechnology · 2026Article
- Functional recovery of the adult murine hippocampus after cryopreservation by vitrification.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Reconstitution of thymopoiesis via implantation of cryopreserved cultured thymus tissue into athymic recipients.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2026Article
- Cryopreservation Strategies to Improve Access to Organ Transplantation.Transplantation · 2026Review
- Nanomedicine in Organ Transplantation: From Graft Preservation and Repair to Immunomodulation and Monitoring.Theranostics · 2026Review
- Advanced Rewarming Technologies for Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Cryopreservation of human cortical organoids using vitrification.Frontiers in molecular neuroscience · 2026Article
- Physical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation.Nature communications · 2025Article
- Dielectric properties of individual cryoprotective agents and cocktails VS55, M22, DP6 at subzero temperatures for cryopreservation.Scientific reports · 2025Article
- 55 MHz constant field dielectric warming of kidneys and ovaries cryopreserved by vitrification.Cryobiology · 2025Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.Scientific reports · 2025Article
- Ischemia Reperfusion Injury Induced Systemic Inflammatory Response Following Interspecies Liver Transplantation.Hepatic medicine : evidence and research · 2025Article
- Beyond the icebox: modern strategies in organ preservation for transplantation.Clinical transplantation and research · 2024Review
- Enhanced VCA Storage: A Pilot Study Demonstrating Supercooling in Orthotopic Rodent Hindlimb Transplantation.Transplantation proceedings · 2024Article
- Optimized partial freezing protocol enables 10-day storage of rat livers.Scientific reports · 2024Article
- Current State and Challenges of Tissue and Organ Cryopreservation in Biobanking.International journal of molecular sciences · 2024Review
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Liver cryopreservation has the potential to enable indefinite organ banking. This study investigated vitrification-the ice-free cryopreservation of livers in a glass-like state-as a promising alternative to conventional cryopreservation, which uniformly fails due to damage from ice formation or cracking. Our unique "nanowarming" technology, which involves perfusing biospecimens with cryoprotective agents (CPAs) and silica-coated iron oxide nanoparticles (sIONPs) and then, after vitrification, exciting the nanoparticles via radiofrequency waves, enables rewarming of vitrified specimens fast enough to avoid ice formation and uniformly enough to prevent cracking from thermal stresses, thereby addressing the two main failures of conventional cryopreservation. This study demonstrates the ability to load rat livers with both CPA and sIONPs by vascular perfusion, cool them rapidly to an ice-free vitrified state, and rapidly and homogenously rewarm them. While there was some elevation of liver enzymes (Alanine Aminotransferase) and impaired indocyanine green (ICG) excretion, the nanowarmed livers were viable, maintained normal tissue architecture, had preserved vascular endothelium, and demonstrated hepatocyte and organ-level function, including production of bile and hepatocyte uptake of ICG during normothermic reperfusion. These findings suggest that cryopreservation of whole livers via vitrification and nanowarming has the potential to achieve organ banking for transplant and other biomedical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.