Evidence map›Paper›PMID 36183022›Full record

ArticleBiotechnology letters2022

Optimization of an adenovirus-vectored zoster vaccine production process with chemically defined medium and a perfusion system.

Jianqi Nie, Yang Sun, He Ren, Lingling Huang, Kai Feng, Ye Li, Zhonghu Bai

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jianqi NieThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Yang SunInstitute of Microbial Engineering, School of Life Sciences, Henan University, Kaifeng, 475004, China.
He RenThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Lingling HuangInstitute of Microbial Engineering, School of Life Sciences, Henan University, Kaifeng, 475004, China.
Kai FengInstitute of Microbial Engineering, School of Life Sciences, Henan University, Kaifeng, 475004, China.
Ye LiNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, China. yeli0622@jiangnan.edu.cn.
Zhonghu BaiNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, China. baizhonghu@jiangnan.edu.cn.ORCID http://orcid.org/0000-0003-3985-7434
Jiangnan University · CNHenan University · CN

Funding

National Key Research and Development Program of China 2018YFA0900804National Natural Science Foundation of China 22108100Natural Science Foundation of Jiangsu Province BK20210472the national first-class discipline program of Light Industry Technology and Engineering LITE2018-24
6 · The paper itself

Abstract

objectivesCells grown in chemically defined medium are sensitive to shear force, potentially resulting in decreased cell growth. We optimized the perfusion process for HEK293 cell-based recombinant adenovirus-vectored zoster vaccine (Ad-HER) production with chemically defined medium.

methodsWe first studied the pseudo-continuous strategies in shake flasks as a mimic of the bioreactor equipped with perfusion systems. Using design of experiment (DoE) in shake flasks, we obtained the regression models between Ad-HER titer/virus input-output ratio and three production process parameters: time of infection (TOI), multiplicity of infection (MOI), and virus production pH (pH). We then confirmed the effect of Pluronic F68 (PF-68) at 3.0 g/L on HEK293 cell growth and Ad-HER production in shake flasks and a 2 L benchtop bioreactor.

resultsThe optimized process was scale-up to a 2 L benchtop bioreactor with the PATFP perfusion system, which yielded cell density of 7.4 × 10

conclusionThis optimization strategy could be used to develop a robust process with stable cell culture performance and adenovirus titer. Increasing PF-68 concentration in chemically defined medium could protect cells from shear stress generated by perfusion system.

Indexed as

Herpes Zoster VaccineAdenoviridaeBioreactorsCell Culture TechniquesHEK293 CellsHumansPerfusionHerpes Zoster VaccineAdenovirusChemically defined mediumPerfusion cultivationShear forceZoster vaccine

Identifiers

PMID36183022
PMCPMC9526465
OpenAlexW4298325711

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.