Evidence map›Paper›PMID 36180636›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Regulatory T-Cell Phenotyping Using CyTOF.

Hugo Barcenilla, Mikael Pihl, Florence Sjögren, Louise Magnusson, Rosaura Casas

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Targeting T regulatory (TCancer drug resistance (Alhambra, Calif.) · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Hugo BarcenillaDepartment of Biomedical and Clinical Sciences, Division of Children's and Women's Health, Faculty of Health Sciences, Linköping University, Linköping, Sweden. hugo.barcenilla@liu.se.
Mikael PihlCore Facility, Flow cytometry Unit, Linköping University, Linköping, Sweden.
Florence SjögrenCore Facility, Flow cytometry Unit, Linköping University, Linköping, Sweden.
Louise MagnussonDepartment of Biomedical and Clinical Sciences, Division of Children's and Women's Health, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
Rosaura CasasDepartment of Biomedical and Clinical Sciences, Division of Children's and Women's Health, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
Linköping University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Regulatory T cells are an important component of the immune system that plays a key role in maintaining homeostasis. Identification of distinct regulatory T cell subsets is essential to understand their function. Mass cytometry or CyTOF is a technology that enables the simultaneous measurement of up to 50 markers in single cells by using antibodies tagged with heavy metals, which are then detected with time-of-flight mass spectrometry. This chapter describes a mass cytometry approach for phenotypic characterization of regulatory T cells and determination of their master transcription factor Foxp3.

Indexed as

Metals, HeavyT-Lymphocytes, RegulatoryFlow CytometryForkhead Transcription FactorsImmunophenotypingT-Lymphocyte SubsetsForkhead Transcription FactorsMetals, HeavyCyTOFFoxp3immunophenotypingMass cytometryphenotypingRegulatory T cellsSingle-cell analysisTreg

Identifiers

PMID36180636
OpenAlexW4298124757

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.