Target-mediated competitive hybridization of hairpin probes for kanamycin detection based on exonuclease III cleavage and DNAzyme catalysis.

Jiafeng Pan, Fang Deng, Lingwen Zeng, Zhi Liu, Junhua Chen

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Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Jiafeng Pan *College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.

Fang Deng *College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.

Lingwen ZengGuangdong Langyuan Biotechnology Co., LTD, Foshan, 528313, China.

Zhi LiuCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China. tigerzhiliu@126.com.

Junhua ChenNational-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management, Institute of Eco-Environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou, 510650, China. 222chenjunhua@163.com.ORCID http://orcid.org/0000-0001-9450-7272

Funding

Foshan Science and Technology Innovation Team Project 2018IT100343the Guangdong Basic and Applied Basic Research Foundation 2019B1515210019the Guangdong Basic and Applied Basic Research Foundation 2021A1515012192the Key Research and Development Projects of Hubei Province 2020BBA053Wuhan Science and Technology Plan Project 2020030603012347

6 · The paper itself

Abstract

Based on aptamer recognition and target-mediated competitive hybridization of hairpin probes, we developed a fluorescence sensor for kanamycin (KAN) detection. The aptamer and KAN binding will open hairpin H1 to release the trigger DNA fragment, which can initiate the competitive hybridization between hairpins H2 and H3. Then, exonuclease III (Exo III) can cleave H2 and H3 to produce numerous DNA3 and DNA4. Through the synergetic hybridization among DNA1, DNA2, DNA3, and DNA4, an active Mg

Indexed as

DNA, CatalyticCatalysisExodeoxyribonucleasesKanamycinOligonucleotidesReproducibility of ResultsDNA, Catalyticexodeoxyribonuclease IIIExodeoxyribonucleasesKanamycinOligonucleotidesAntibioticAptamerCompetitive hybridizationDNAzymeExonuclease IIIKanamycin

Identifiers

PMID36178489

DOI10.1139/cjc-2013-0285

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.