ArticleDevelopment (Cambridge, England)2022
Single-nuclei transcriptome analysis of the shoot apex vascular system differentiation in Populus.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review of single-cell RNA sequencing applications and insights in Oryza sativa response to environmental stresses.Molecular biology reports · 2025Pooled it
- Evolution of molecular networks underlying plant tissue patterning: insights from conducting tissues.Journal of experimental botany · 2026Review
- Single-nucleus transcriptomics resolves multiple fate dynamics between inflorescence meristem and primary stem.Science advances · 2026Article
- Spatial and single-cell transcriptomics uncover brassinosteroid-mediated coordination of sepal elongation in Arabidopsis.Genome biology · 2026Article
- Single-cell and spatial transcriptomics in Phragmites australis reveal the association of B chromosomes with plant invasiveness.Genome biology · 2026Article
- Single Cell Multiomics of Hooked Potato Stolons Reveals Parallels to Shoot Apical Meristems in Arabidopsis.Plant biotechnology journal · 2026Article
- Endogenous Multilayer Control of Cambial Stem Cells and Its Consequences for Wood Formation.Plants (Basel, Switzerland) · 2026Review
- A cell-type-specific regulon controlling monoterpene indole alkaloid biosynthesis with feedback and feedforward activation loops.The New phytologist · 2026Article
- Large-scale single-cell profiling of stem cells identifies redundant regulators of shoot development and yield trait variation.Developmental cell · 2025Article
- Single-nuclei sequencing of Moricandia arvensis reveals bundle sheath cell function in the photorespiratory shuttle of C3-C4 intermediate Brassicaceae.Journal of experimental botany · 2025Article
- Integrated single-cell transcriptomics and spatial metabolomics unveil cellular differentiation and ginsenosides biosynthesis inHorticulture research · 2025Article
- Deep tissue profiling of Populus stem at single nucleus level reveals uncharacterized cell types and cell-specific gene regulatory networks.Genome biology · 2025Article
- Genome-wide association studies in forestry.Molecular biology reports · 2025Review
- Brassinosteroids mediate proper coordination of sepal elongation.bioRxiv : the preprint server for biology · 2025Article
- Validation of Fiber-Dominant Expressing Gene Promoters inPlants (Basel, Switzerland) · 2025Article
- Developmental landscape and asymmetric gene expression in the leaf vasculature ofHorticulture research · 2025Article
- Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics.The New phytologist · 2025Article
- Investigating biological nitrogen fixation via single-cell transcriptomics.Journal of experimental botany · 2025Review
- Orthologous marker groups reveal broad cell identity conservation across plant single-cell transcriptomes.Nature communications · 2025Article
- Single-cell RNA sequencing of shoot apex reveals the mechanism of cyclin regulating cell division via auxin signaling pathway inFrontiers in plant science · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiation of stem cells in the plant apex gives rise to aerial tissues and organs. Presently, we lack a lineage map of the shoot apex cells in woody perennials - a crucial gap considering their role in determining primary and secondary growth. Here, we used single-nuclei RNA-sequencing to determine cell type-specific transcriptomes of the Populus vegetative shoot apex. We identified highly heterogeneous cell populations clustered into seven broad groups represented by 18 transcriptionally distinct cell clusters. Next, we established the developmental trajectories of the epidermis, leaf mesophyll and vascular tissue. Motivated by the high similarities between Populus and Arabidopsis cell population in the vegetative apex, we applied a pipeline for interspecific single-cell gene expression data integration. We contrasted the developmental trajectories of primary phloem and xylem formation in both species, establishing the first comparison of vascular development between a model annual herbaceous and a woody perennial plant species. Our results offer a valuable resource for investigating the principles underlying cell division and differentiation conserved between herbaceous and perennial species while also allowing us to examine species-specific differences at single-cell resolution.
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