Evidence map›Paper›PMID 36176723›Full record

ArticleTransplantation direct2022

Luminal Preservation Protects the Small Intestine in a Brain-dead Rat Model.

Guido Trentadue, Leandro Vecchio, Gursah Kats-Ugurlu, Julieta Vernengo, Jan Willem Haveman, Ivana Ivanoff, Klaas Nico Faber, Martín Rumbo, Gerard Dijkstra

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transplantation direct, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Guido TrentadueDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen. Groningen, The Netherlands.
Leandro VecchioInstitute of Physiopathological Immunological Studies, Laboratory of Organ and Tissue Transplant, National University of La Plata. La Plata, Argentina.
Gursah Kats-UgurluDepartment of Pathology, University Medical Center Groningen, University of Groningen. Groningen, The Netherlands.
Julieta VernengoInstitute of Physiopathological Immunological Studies, Laboratory of Organ and Tissue Transplant, National University of La Plata. La Plata, Argentina.
Jan Willem HavemanDepartment of Surgery, University Medical Center Groningen, University of Groningen. Groningen, The Netherlands.
Ivana IvanoffInstitute of Physiopathological Immunological Studies, Laboratory of Organ and Tissue Transplant, National University of La Plata. La Plata, Argentina.
Klaas Nico FaberDepartment of Laboratory Medicine, University Medical Center Groningen, University of Groningen. Groningen, The Netherlands.
Martín RumboInstitute of Physiopathological Immunological Studies, Laboratory of Organ and Tissue Transplant, National University of La Plata. La Plata, Argentina.
Gerard DijkstraDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen. Groningen, The Netherlands.
University Medical Center Groningen · NLBiochemistry Research Institute of La Plata · AR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intestinal transplantation depends on donation after brain death (DBD). Luminal preservation (LP) has been beneficial against preservation injury in previous studies in animal models, but none include DBD. This study aims to investigate whether these benefits occur also with DBD. Methods: Wistar rats (male, N = 9) underwent brain death for 2 h. Thereafter, vascular perfusion was done with University of Wisconsin solution (UW). The small intestine was then explanted and randomized into 3 groups: control (empty segment), LP+PEG (with polyethylene glycol 3350 solution), or LP+UW (with UW), treated and tied shut. Ice-cold UW was used for cold storage. Samples were taken at procurement and after 4 (t = 4) and 8 h (t = 8) of preservation. Histopathological scorings were performed for intestinal preservation injury, subepithelial space, absence of epithelial lining, and hemeoxygenase-1 expression. Results: There was low-level mucosal injury (median intestinal preservation injury score 2) at procurement. At t = 4, bowels treated without LP had more damage than LP-treated samples (control score 4, LP+PEG 2 and LP+UW 2, Conclusions: Luminal perfusion of the small intestine with UW or PEG protects the mucosa in brain-dead rats for up to 4 h. Fewer benefits of LP were found than previously described in non-DBD models. To mimic the clinical situation, DBD should be included in future animal studies on intestinal preservation.

Identifiers

PMID36176723
PMCPMC9514830
OpenAlexW4297093298

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.