ArticleFrontiers in nutrition2022
Comparative study of the most commonly used methods for total protein determination in milk of different species and their ultrafiltration products.
Article in Frontiers in nutrition, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
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- T16 modulated extracellular matrix remodeling in fibroblasts via paracrine activation of TGF-β1 through M2 macrophage polarization.Molecular biology reports · 2025Article
- Immobilization of Lipase fromMolecules (Basel, Switzerland) · 2024Article
- Comparative analysis of different methods for protein quantification in donated human milk.Frontiers in pediatrics · 2024Article
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- Microfluidic Analysis for the Determination of Protein Content in Different Types of Plant-Based Drinks.Molecules (Basel, Switzerland) · 2023Article
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- Isolation, identification, and characterization of corn-derived antioxidant peptides from corn fermented milk byFrontiers in nutrition · 2022Article
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3 authors.
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Abstract
Milk ultrafiltration is a widely used membrane filtration process that allows the recuperation of whey proteins in a concentrate high in total solids, which can later be transformed in multiple healthy dairy products with great prospects for the food industry. Protein content is a decisive factor for the technological performance of milk concentrates and currently, the ISO standard method for its determination is Kjeldahl, which is time-consuming and requires specific instrumentation. For this reason, the use of rapid methods to quantify protein would greatly facilitate the monitoring of the milk ultrafiltration process. In this study, the bicinchoninic acid assay (BCA), the detergent compatible Bradford assay and the Dumas method were compared to Kjeldahl protein determination to select a quick and accurate methodology suitable for milk of different species and its ultrafiltration products (retentates and permeates). The protein content obtained from Bradford assay and Dumas method in origin milk and retentate samples was consistent with Kjeldahl values. In contrast, BCA protein levels were significantly different when compared to Kjeldahl and no method was proved to be suitable for protein determination in permeate samples. The use of sodium dodecyl sulfate was also examined to improve protein measurements without success. In comparison with the official method, Bradford assay quantitatively provided the best results, and it would be recommended for a quick, economic and easy determination of total protein content in milk and retentate samples.
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