Evidence map›Paper›PMID 36175823›Full record

ArticleMolecular neurobiology2022

Combination Therapy of Mesenchymal Stem Cell Transplantation and Astrocyte Ablation Improve Remyelination in a Cuprizone-Induced Demyelination Mouse Model.

Soheila Madadi, Elham Shiri, Parichehr Pasbakhsh, Fatemeh Tahmasebi, Shokoofeh Kazemzadeh, Kazem Zibara, Iraj Ragerdi Kashani

RetractedErratum issuedAbstract readRetracted Publication
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Astrocytes: Lessons Learned from the Cuprizone Model.International journal of molecular sciences · 2023
    Review
  5. Remyelination in Multiple Sclerosis: Findings in the Cuprizone Model.International journal of molecular sciences · 2022
    Review
  6. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 2 countries.

Soheila MadadiDepartment of Anatomy, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran.ORCID http://orcid.org/0000-0003-4746-6652
Elham ShiriDepartment of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Parichehr PasbakhshDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Fatemeh TahmasebiDepartment of Anatomy, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Shokoofeh KazemzadehDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Kazem ZibaraPRASE and Biology Department, Faculty of Sciences, Lebanese University, Beirut, Lebanon. kzibara@ul.edu.lb.ORCID http://orcid.org/0000-0002-9887-072X
Iraj Ragerdi KashaniDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. ragerdi@tums.ac.ir.ORCID http://orcid.org/0000-0003-3842-7950
Tehran University of Medical Sciences · IRHamedan University of Medical Sciences · IRJahrom University of Medical Sciences · IRLebanese University · LBMashhad University of Medical Sciences · IR

Funding

Tehran University of Medical Sciences and Health Services 95-04-30-33652
6 · The paper itself

Abstract

Astrocytes display an active, dual, and controversial role in multiple sclerosis (MS), a chronic inflammatory demyelination disorder. However, mesenchymal stem cells (MSCs) can affect myelination in demyelinating disorders. This study aimed to investigate the effect of single and combination therapies of astrocyte ablation and MSC transplantation on remyelination in the cuprizone (CPZ) model of MS. C57BL/6 mice were fed 0.2% CPZ diet for 12 weeks. Astrocytes were ablated twice by L-a-aminoadipate (L-AAA) at the beginning of weeks 13 and 14 whereas MSCs were injected in the corpus callosum at the beginning of week 13. Motor coordination and balance were assessed through rotarod test whereas myelin content was evaluated by Luxol-fast blue (LFB) staining and transmission electron microscopy (TEM). Glial cells were assessed by immunofluorescence staining while mRNA expression was evaluated by quantitative real-time PCR. Combination treatment of ablation of astrocytes and MSC transplantation (CPZ + MSC + L-AAA) significantly decreased motor coordination deficits better than single treatments (CPZ + MSCs or CPZ + L-AAA), in comparison to CPZ mice. In addition, L-AAA and MSCs treatment significantly enhanced remyelination compared to CPZ group. Moreover, combination therapy caused a significant decrease in the number of GFAP

Indexed as

Demyelinating DiseasesMesenchymal Stem Cell TransplantationMultiple SclerosisRemyelinationAnimalsAstrocytesCuprizoneDisease Models, AnimalMiceMice, Inbred C57BLMyelin SheathRNA, MessengerCuprizoneRNA, MessengerAstrocyte ablationCuprizone (CPZ)L-a-aminoadipate (L-AAA)Mesenchymal stem cells (MSCs)Multiple sclerosis (MS)Remyelination

Identifiers

PMID36175823
OpenAlexW4297996152

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.