Evidence map›Paper›PMID 36175639›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Enzymatic Substrates and Fluorescence Imaging of Influenza Virus Sialidase.

Yuuki Kurebayashi, Tadanobu Takahashi, Takashi Suzuki

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Yuuki KurebayashiDepartment of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
Tadanobu TakahashiDepartment of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
Takashi SuzukiDepartment of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan. suzukit@u-shizuoka-ken.ac.jp.
University of Shizuoka · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunostaining with an antiviral antibody is usually performed to visualize virus-infected cells. In contrast, this study established an easy method for fluorescence (FL) imaging of cells infected with influenza A and B viruses and some paramyxoviruses without the need for cell fixation and an antiviral antibody. These viruses and the cells they have infected express the viral surface enzymes "neuraminidase" or "hemagglutinin-neuraminidase," which show sialidase activity. Sialidase activity is fluorescently visualized by using a sialidase fluorogenic probe developed in our previous study. The probe enables histochemical FL imaging of the virus-infected cells and applies to virus isolation and detection of an influenza virus resistant to sialidase inhibitors anti-influenza drugs.

Indexed as

Influenza, HumanOrthomyxoviridaeAntibodies, ViralAntiviral AgentsHemagglutininsHumansNeuraminidaseOptical ImagingAntibodies, ViralAntiviral AgentsHemagglutininsNeuraminidaseDrug resistanceFluorescence imagingImaging probeInfected cellsInfluenza virusNeuraminidaseParamyxovirusSialidaseVirus isolation

Identifiers

PMID36175639
OpenAlexW4298087546

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.