Evidence map›Paper›PMID 36175572›Full record

ArticleScientific reports2022

Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators.

Menglu Yang, Haakon K Fjærvoll, Ketil A Fjærvoll, Nicholas H Wang, Tor P Utheim, Charles N Serhan, Darlene A Dartt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Immunomodulation of periodontitis with SPMs.Frontiers in oral health · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Menglu YangDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research Institute, 20 Staniford Street, Boston, MA, 02114, USA.
Haakon K FjærvollDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research Institute, 20 Staniford Street, Boston, MA, 02114, USA.
Ketil A FjærvollDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research Institute, 20 Staniford Street, Boston, MA, 02114, USA.
Nicholas H WangDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research Institute, 20 Staniford Street, Boston, MA, 02114, USA.
Tor P UtheimDepartment of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
Charles N SerhanDepartment of Anesthesia, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.ORCID 0000-0003-4627-8545
Darlene A DarttDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research Institute, 20 Staniford Street, Boston, MA, 02114, USA. Darlene_dartt@meei.harvard.edu.
Massachusetts Eye and Ear Infirmary · USBrigham and Women's Hospital · USOslo University Hospital · NO

Funding

VISION RESEARCHP30EY003790 · NEI · SCHEPENS EYE RESEARCH INSTITUTE · PI Patricia Ann D'Amore · 1985 to 2026
$25.5M
Conjunctival Goblet Cell Mucin Secretion in Inflammation and Its ResolutionR01EY019470 · NEI · SCHEPENS EYE RESEARCH INSTITUTE · PI DARTT, DARLENE A · 2009 to 2023
$7.4M
NEI NIH HHS P30 EY003790NEI NIH HHS R01 EY019470
6 · The paper itself

Abstract

Many conjunctival inflammatory diseases differ between the sexes and altered conjunctival goblet cells (CGCs) response is often involved. Inflammation is initiated by the release of pro-inflammatory mediators and terminated by the biosynthesis of specialized pro-resolution mediators (SPMs). Herein, we determined the sex-based difference in the responses of CGCs to inflammatory stimuli or pro-resolving lipid SPMs and their interaction with sex hormones. GCs were cultured from pieces of human conjunctiva in RPMI media. CGCs were transferred 24 h before the start of experiments to phenol red-free and FBS-free media to minimize exogenous hormones. RT-PCR, immunofluorescence microscopy (IF), and Western Blot (WB) were performed to determine the presence of sex hormone receptors. Cellular response to pro-inflammatory stimuli or SPMs was studied by measuring the increase in intracellular [Ca

Indexed as

Conjunctival DiseasesLipoxinsCarbacholConjunctivaDihydrotestosteroneEstradiolEstrogen Receptor alphaEstrogen Receptor betaEstroneFemaleFura-2Goblet CellsHistamineHumansLeukotrienesMaleCarbacholDihydrotestosteroneEstradiolEstrogen Receptor alphaEstrogen Receptor betaEstroneFura-2HistamineLeukotrienesLipoxinsReceptors, AndrogenReceptors, Estrogen

Identifiers

PMID36175572
PMCPMC9522727
OpenAlexW4297998251

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.