ArticleNPJ science of food2022
Spent media analysis suggests cultivated meat media will require species and cell type optimization.
Article in NPJ science of food, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- MuSC cultivation for cultured meat production: recent advances, current limitations, and future directions.Food science of animal resources · 2026Review
- Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.Biotechnology and bioengineering · 2026Review
- Enzymatically triggered glucose-releasing scaffolds as nutritional supports for millimeter-scale tissue engineering.Materials today. Bio · 2026Article
- Critical regulatory pathways for the development of sheep myoblasts and the enhancement of cultured meat quality.Food chemistry: X · 2026Article
- Hastening meat production: a review of growth promoters for traditional and cultured meat production.Journal of animal science and technology · 2026Review
- Myogenic Potential inACS omega · 2026Article
- Cell sources and engineering strategies for cultured meat production: a critical comparative review.Frontiers in nutrition · 2026Review
- Bioprocess Engineering for Cultivated Meat.Advances in biochemical engineering/biotechnology · 2026Review
- Development of Cell Culture Media for Cultivated Meat Production.Advances in biochemical engineering/biotechnology · 2026Review
- Bioreactor Design and Engineering for Cultivated Meat Manufacturing.Advances in biochemical engineering/biotechnology · 2026Review
- Multi-response optimization of defined and undefined serum-free media for enhanced proliferation of immortalized lamb muscle cells.Scientific reports · 2025Article
- Dynamic shifts in metabolic demand during myogenic progression in porcine skeletal muscle stem cells.NPJ science of food · 2025Article
- Proliferation and metabolic activity of the Atlantic sturgeon cell line AOXlar7y under short-term serum-reduced conditions, and the effect of stimulation with growth factors and cytokines.Frontiers in toxicology · 2025Article
- Development and optimization of an ammonia removal strategy for sustainable recycling of cell culture spent media in cultivated meat production: from concept to implementation.Frontiers in bioengineering and biotechnology · 2025Article
- Effect of Stem Cells from Human Exfoliated Deciduous Teeth Spent Medium (SHED-SM) on the Healing of Traumatic Buccal Mucosal Ulcers.F1000Research · 2025Article
- Effect of the addition of sulfated polysaccharides of animal and algal origin in the freezing medium ofAnimal reproduction · 2025Article
- Serum markers for beef meat quality: Potential media supplement for cell-cultured meat production.Current research in food science · 2025Review
- Exploring cost reduction strategies for serum free media development.NPJ science of food · 2024Review
- A scoping review of cultivated meat techno-economic analyses to inform future research directions for scaled-up manufacturing.Nature food · 2024Article
- Cell-cultivated aquatic food products: emerging production systems for seafood.Journal of biological engineering · 2024Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Cell culture media design is perhaps the most significant hurdle currently facing the commercialization of cultivated meat as an alternative source of dietary protein. Since media optimization for a specific culture system requires a significant amount of effort and investment, a major question remaining is whether media formulations can be easily shared across multiple production schemes for cells of different species and lineages. Here, we perform spent medium analysis to compare the specific nutrient utilization of primary embryonic chicken muscle precursor cells and fibroblasts to the murine C2C12 myoblast cell line. We demonstrate that these related cell types have significantly different nutrient utilization patterns collectively and on a per-cell basis, and that many components of conventional media do not appear to be depleted by the cells. Namely, glucose was not consumed as rapidly nor as completely by the chicken muscle precursors compared to other cells overall, and there were significant differences in specific consumption rates for several other key nutrients over the first day of culture. Ultimately, our results indicate that no one medium is likely ideal and cost effective to culture multiple cell types and that novel methods to streamline media optimization efforts will be important for the industry to develop.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.