Evidence map›Paper›PMID 36174976›Full record

ArticleJournal of veterinary science2022

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus.

Yuying Cao, Li Yuan, Shunli Yang, Youjun Shang, Bin Yang, Zhizhong Jing, Huichen Guo, Shuanghui Yin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yuying CaoState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID https://orcid.org/0000-0003-1886-1098
Li YuanState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID https://orcid.org/0000-0002-3562-9947
Shunli YangState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID https://orcid.org/0000-0001-5937-0004
Youjun ShangState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID https://orcid.org/0000-0001-8016-0511
Bin YangState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID https://orcid.org/0000-0002-7883-0739
Zhizhong JingState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID https://orcid.org/0000-0003-1171-7931
Huichen GuoState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID https://orcid.org/0000-0001-8074-3872
Shuanghui YinState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. yinshuanghui@caas.cn.ORCID https://orcid.org/0000-0001-7276-9140
Lanzhou Veterinary Research Institute · CNYangtze University · CN

Funding

Agricultural Science and Technology Innovation Program CAAS-ASTIP-2014-LVRI-09
6 · The paper itself

Abstract

backgroundClassical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry.

objectivesThis study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples.

methodsA spbELISA method was developed based on the recombinant E2 expressed in

resultsThe spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies.

conclusionsThe spbELISA has practical applications in assessing the vaccination status of large pig herds.

Indexed as

Classical Swine FeverClassical Swine Fever VirusSwine DiseasesAnimalsAntibodiesEnzyme-Linked Immunosorbent AssayEscherichia coliFluorescent Antibody Technique, IndirectSwineAntibodiesantibody detectionblocking ELISAClassical swine fever virusE2 protein

Identifiers

PMID36174976
PMCPMC9523335
OpenAlexW4293195338

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.