Evidence map›Paper›PMID 36174734›Full record

ArticleJournal of immunological methods2022

A novel method for in vitro culture and expansion of nonhuman primate B cells.

Isabel DeLaura, Paul M Schroder, Janghoon Yoon, Joseph Ladowski, Imran J Anwar, Brian Ezekian, Robin Schmitz, Zachary W Fitch, Jean Kwun, Stuart J Knechtle

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunological methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Isabel DeLauraDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Paul M SchroderDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Janghoon YoonDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Joseph LadowskiDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Imran J AnwarDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Brian EzekianDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Robin SchmitzDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Zachary W FitchDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Jean KwunDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA. Electronic address: jean.kwun@duke.edu.
Stuart J KnechtleDuke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, USA. Electronic address: stuart.knechtle@duke.edu.
Duke Medical Center · US

Funding

Transplant Tolerance in Non-Human PrimatesU19AI051731 · NIAID · UNIVERSITY OF WESTERN ONTARIO · PI LARSEN, CHRISTIAN P. · 2002 to 2022
$54.1M
The Risks and Opportunities of Homeostatic RepopulationU19AI131471 · NIAID · DUKE UNIVERSITY · PI Andrew Serghios Barbas · 2017 to 2026
$30.4M
Basic Immunology Training ProgramT32AI052077 · NIAID · DUKE UNIVERSITY · PI KRANGEL, MICHAEL S · 2002 to 2024
$4.2M
NIAID NIH HHS U19 AI051731NIAID NIH HHS U19 AI131471
6 · The paper itself

Abstract

backgroundGiven the role of B cells in sensitization and antibody-mediated rejection pathogenesis, the ability to identify, isolate, and study B cells in vitro is critical for understanding these processes and developing novel therapeutics. While in vivo nonhuman primate models have been used to this end, an in vitro nonhuman primate model of B cell activation and proliferation has not been developed.

methodsCD20

resultsThe optimized culture system consisted of MS40L cells co-cultured with B and allogenic T cells and stimulated with cytokines. This culture system resulted in increased memory cells and plasmablasts over time compared to other culture systems. Comparison of culture of naïve and sensitized nonhuman primate samples revealed faster B cell exhaustion and marginally increased plasmablast differentiation in sensitized culture. Donor-specific antibody production was not observed in either culture group.

conclusionsThis study describes the first in vitro nonhuman primate model of B cell activation and proliferation using both naïve and allosensitized samples. This model provides an opportunity for exploration of B cell mechanisms and novel therapeutics and is a preliminary step in the development of an in vitro germinal center model.

Indexed as

AnimalsHumansMiceAlloantibodyB cell cultureCell cultureNonhuman primatePlasmablast

Identifiers

PMID36174734
PMCPMC10486248
OpenAlexW4297141531

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.