ArticleGenome biology and evolution2022
Long-read Sequencing Data Reveals Dynamic Evolution of Mitochondrial Genome Size and the Phylogenetic Utility of Mitochondrial DNA in Hercules Beetles (Dynastes; Scarabaeidae).
Article in Genome biology and evolution, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 51 citations in OpenAlex.
- De Novo Assembly and Comparative Mitochondrial Genome Analysis of Three Salt Marsh Specialist Ground Beetle Species (Coleoptera: Carabidae).Ecology and evolution · 2026Article
- Towards a mitogenomic phylogeny of mud dragons (Kinorhyncha): two new mitogenomes from the Pycnophyidae family.Scientific reports · 2026Article
- Chromosome-Level Reference Genome of an Endemic, Endangered Long-Armed Scarab (Ecology and evolution · 2026Article
- Mitochondrial genomes and phylogeny of Atratus and Educator Group species of the Melanoconion Section of Culex (Melanoconion) (Diptera: Culicidae)Memorias do Instituto Oswaldo Cruz · 2026Article
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- The complete mitochondrial genome of the parasitoidMitochondrial DNA. Part B, Resources · 2026Article
- The Mitochondrial Genome of Melipona fasciculata (Apidae, Meliponini): Genome Organization and Comparative Analyses, Phylogenetic Implications and Divergence Time Estimations.Biochemical genetics · 2025Article
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- A reference genome enhances the power to detect signatures of recent anthropogenic impact in genomic data: a lesson learned from a stag beetle system.BMC biology · 2025Article
- Cytogenetic and Molecular Characterization ofInsects · 2025Article
- Comparative analysis of the mitochondrial genomes of the soft-shelled turtles Palea steindachneri and Pelodiscus axenaria and phylogenetic implications for Trionychia.Scientific reports · 2025Article
- High-Throughput Sequencing Enables Rapid Analyses of Nematode Mitochondrial Genomes from an Environmental Sample.Pathogens (Basel, Switzerland) · 2025Article
- Integrating Wing Morphometrics and Mitogenomic Sequences Supports Species-level Distinction betweenZoological studies · 2025Article
- The first complete mitochondrial genome of aZooKeys · 2025Article
- A reproducible workflow for assembling the mitochondrial genome ofEcology and evolution · 2024Article
- Evolution of myxozoan mitochondrial genomes: insights from myxobolids.BMC genomics · 2024Article
- Annotated genome and transcriptome of the endangered Caribbean mountainous star coral (Orbicella faveolata) using PacBio long-read sequencing.BMC genomics · 2024Article
- Complete mitochondrial genome of the Satanas beetle,Mitochondrial DNA. Part B, Resources · 2024Article
- Complete mitochondrial genome ofZooKeys · 2024Article
- First report of the complete mitochondrial genome of 3 beetles (Coleoptera: Scarabaeidae) harming Gastrodia elata (Asparagales: Orchidaceae).Journal of insect science (Online) · 2024Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The evolutionary dynamics and phylogenetic utility of mitochondrial genomes (mitogenomes) have been of particular interest to systematists and evolutionary biologists. However, certain mitochondrial features, such as the molecular evolution of the control region in insects, remain poorly explored due to technological constraints. Using a combination of long- and short-read sequencing data, we assembled ten complete mitogenomes from ten Hercules beetles. We found large-sized mitogenomes (from 24 to 28 kb), which are among the largest in insects. The variation in genome size can be attributed to copy-number evolution of tandem repeats in the control region. Furthermore, one type of tandem repeat was found flanking the conserved sequence block in the control region. Importantly, such variation, which made up around 30% of the size of the mitogenome, may only become detectable should long-read sequencing technology be applied. We also found that, although different mitochondrial loci often inferred different phylogenetic histories, none of the mitochondrial loci statistically reject a concatenated mitochondrial phylogeny, supporting the hypothesis that all mitochondrial loci share a single genealogical history. We on the other hand reported statistical support for mito-nuclear phylogenetic discordance in 50% of mitochondrial loci. We argue that long-read DNA sequencing should become a standard application in the rapidly growing field of mitogenome sequencing. Furthermore, mitochondrial gene trees may differ even though they share a common genealogical history, and ND loci could be better candidates for phylogenetics than the commonly used COX1.
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