Evidence map›Paper›PMID 36173578›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Targeted DNA Methylation in Mouse Early Embryos.

Taiga Yamazaki, Yu Hatano, Noritada Kobayashi, Kazuo Yamagata

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Taiga YamazakiDivision of Biomedical Research, Kitasato University Medical Center, Kitasato University, Kitamoto, Saitama, Japan. tyamazak@insti.kitasato-u.ac.jp.
Yu HatanoFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.
Noritada KobayashiDivision of Biomedical Research, Kitasato University Medical Center, Kitasato University, Kitamoto, Saitama, Japan.
Kazuo YamagataFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan. yamagata@waka.kindai.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In germ cell lines, including early preimplantation embryos, centromeres and pericentromeres are known to show a marked hypomethylation pattern compared to somatic cells. Elucidation of the biological function of this region-specific DNA hypomethylation state, region-specific epigenomic manipulation is essential as an analytical method. We have applied genome editing to show that region-specific DNA methylation can be effectively introduced by a fusion protein, TALE, which recognizes pericentromeres, and SssI, a bacterial CpG methyltransferase. This makes it possible to increase the DNA methylation state of the pericentromeres, which is normally about 20%, to about 60-75%, enabling comparative analysis of the developmental processes of normal embryos with hypomethylated pericentromeres and embryos that have been epigenetically edited to be hypermethylated. In this chapter, we describe a method for introducing DNA methylation into pericentromeres of early mouse embryos by expressing TALE-SssI fusion protein and a method for detecting DNA methylation.

Indexed as

BlastocystDNA MethylationAnimalsDNAGene EditingMethyltransferasesMiceDNAMethyltransferasesCpG methyltransferaseDNA methylationEpigenome editingLive-cell imagingPericentromerePreimplantation embryosTALE

Identifiers

PMID36173578

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.